TITLE:
Nutrients Modulate T1r2 Transcript Levels in MIN 6 and Primary Cultured Taste Buds Cells under High Glucose Condition
AUTHORS:
Shin-ichi Nakmura, Tetsuya Ookura
KEYWORDS:
Glucose, T1r2, MIN6, Primary Culture, Taste Buds
JOURNAL NAME:
Food and Nutrition Sciences,
Vol.7 No.4,
April
28,
2016
ABSTRACT: The sweet taste receptors comprised of T1r2 and T1r3, sense glucose concentrations in the gastrointestine.
While hyperglycemia was reported to decrease the T1R2 and T1R3 tanscript levels
in healthy subjects, no change was observed in type 2 diabetes patients. We investigated which
glucose level and nutrients affect those transcript levels in MIN 6 and primary cultured taste buds
cells using quantitative Reverse Trancription Polymerase Chain Reaction. High glucose diminished
T1r2 transcript levels in MIN 6 and primary cultured taste buds cells. Resveratrol and its
analogue augmented transcript levels of T1r1 and T1r2 above normal levels in MIN 6 cells in the
medium with 25 mM glucose. Adenine, but not guanine, augmented T1r2 transcript levels of MIN 6
cells in the medium with 25 mM glucose. These results imply that nutrients in meals could affect
sweet taste sensitivity by modulating T1r2 transcript levels in response to blood glucose levels.