TITLE:
A New Evaluation Method for Antibiotic-Resistant Bacterial Groups in Environment
AUTHORS:
Katsuji Watanabe, Naoto Horinishi, Kunimasa Matsumoto, Akihiro Tanaka, Kenichi Yakushido
KEYWORDS:
Colistin Resistant Bacteria, Chlortetracycline Resistant Bacteria, Multi Drug Resistant Bacteria, Multiple Enzyme Restriction Fragment Length Polymorphism Analysis, The Most Probable Number Method, Microchip Electrophoresis System
JOURNAL NAME:
Advances in Microbiology,
Vol.6 No.3,
March
11,
2016
ABSTRACT: In the present manuscript
it was presented whether spreading of antibiotic resistant bacterial groups in environment
could be monitored by our newly developed method by enumerating antibiotic resistant
bacterial groups in various biological wastes and composts. Although the numbers
were not so high, diverse kinds of colistin resistant bacteria (25 mg·L-1) were included in row cattle feces (1.78 × 104 MPN
g-1) and cattle feces manure (>3.84 × 104 MPN g-1).
Compost originated from leftover food (>44.8 × 104 MPN g-1)
and shochu lee (>320 × 104 MPN g-1) included higher numbers
of chlortetracycline resistant Pseudomonas sp., (25 mg·L-1), and row cattle feces included higher numbers of chlortetracycline
resistant Enterobacteriacea (15.7 × 104 MPN g-1), which mostly
consisted from Pantoea sp. or Xenorhobdus doucetiae. Numbers of multi drug
resistant bacteria, resistant to 25 mg·L-1 of ciprofloxacin, streptomycin,
chloramphenicol, and ampicillin, were the highest in row cattle feces (>143.6
× 104 MPN g-1), followed by cattle feces manure (4.19 × 104 MPN g-1), and shochu lee (0.36 × 104 MPN g-1),
which included diverse kinds of bacterial group. The present results indicated that
higher numbers of multi drug resistant bacteria were typically found in row cattle
feces, and the method was found suitable to enumerate and identify them. These results
suggested that the method might become their environmental risk evaluation method.