TITLE:
First Detection of Human Polyomaviruses in HIV Patients with Suspected Neurological Disease in Montería, Colombia
AUTHORS:
Vaneza Tique, Samia Barrera, Salim Mattar, Jorge Miranda, Francisco Camargo
KEYWORDS:
Progressive Multifocal Leukoencephalopathy, Meningitis, Encephalopathy, AIDS
JOURNAL NAME:
Modern Research in Inflammation,
Vol.4 No.3,
February
24,
2016
ABSTRACT: Objective: The objective
is to establish the presence of JC and/or BK polyomavirus in HIV patients with
symptoms of encephalitis and/or meningitis. Methodology: From September 2009 to
December 2011, a prospective study was conducted. 34 HIV patients with symptoms
consistent with encephalitis and/or meningitis were included. The work was
conducted in 3 hospitals in the city of Monteria. Viral DNA extraction was
performed on samples of cerebrospinal fluid (CSF) using a commercial kit
(Quiagen, USA). The detection of BKV and JCV was performed by multiplex real-time
PCR (LightMix?, Roche Diagnostics, Germany) with primers specific for the
short t antigen gene fragment, labeled probes and one internal control.
Results: In 9 (26%) of 34 patients included in the study, JCV virus was
detected; only 1 (3%) patient had coinfection with JCV/BKV. The mortality rate
was 3%. The cytochemical examination of CSF in positive patients presented
average values: 40.7 mg/dL glucose, 171.66 mg/dL protein, 19.8 mm3 leukocytes,
and 109.8 mm3 erythrocytes.
Conclusion: The findings demonstrate that JCV and BKV have a significant
occurrence in HIV patients with CSF infections in Monteria.