TITLE:
Optimization of Hydrocarbons Biodegradation by Bacterial Strains Isolated from Wastewaters in Ouagadougou, Burkina Faso: Case Study of SAE 40/50 Used Oils and Diesel
AUTHORS:
Adama Sawadogo, Harmonie C. Otoidobiga, Léon W. Nitiema, Alfred S. Traoré, Dayéri Dianou
KEYWORDS:
Biodegradation, Hydrocarbon, Nutrient Factor, Surfactant, Bacterial Strain
JOURNAL NAME:
Journal of Agricultural Chemistry and Environment,
Vol.5 No.1,
February
24,
2016
ABSTRACT: Environmental pollution with petroleum and petrochemical products such as diesel and used oils has been recognized as one of the most serious current problem in the world, especially in developing countries. These petrochemical products devastate the soil, surface and underground waters and alter the microbial population at the polluted sites. Thus, the present work aims to optimize the biodegradation of diesel and two used oils (SAE 40 and SAE 50) by bacterial strains namely Acinetobacter S2 and Pseudomonas S7 using either nutrient factors (yeast extract, peptone or trace elements) or surfactants (tween 80 or Sodium Dodecyl Sulfate: SDS). The strains are incubated alone or together with the used oils or diesel supplemented or not with nutrient factors or surfactants for 14, 28, 42 and 56 days, respectively. For all the incubation period, the hydrocarbons degradation rates are determined by gravimetric assay. The results obtained show that nutrient factors increase significantly SAE 50 used oil biodegradation (p = 0.009). Similarly, tween 80 increases SAE 50 and SAE 40 used oils biodegradation but not diesel one. The results also show a significant difference between biodegradation rates at 14, 28, 42 and 56 days for all the hydro-carbons tested (p Acinetobacter S2 and Pseudomonas S7 increases the degradation over the one of the strains alone.