TITLE:
Qualitative Determination of Photodegradation Products of Progesterone and Testosterone in Aqueous Solution
AUTHORS:
Ladji Méité, Baba Donafologo Soro, Narcisse Kouassi Aboua, Véronique Mambo, Karim Sory Traoré, Patrick Mazellier, Joseh De Laat
KEYWORDS:
Photoproducts, Testosterone, Progesterone, GLC/MS, LC/UV-DAD/MS
JOURNAL NAME:
American Journal of Analytical Chemistry,
Vol.7 No.1,
January
19,
2016
ABSTRACT: Direct
photochemical degradations of progesterone (PR) and testosterone (TR), two
naturally produced hormones, have been conducted in non-buffered aqueous
solution (pH ranging between 5.5 and 6.0). The irradiation experiments were
carried out in a batch reactor upon monochromatic (254 nm) and polychromatic (λ > 290 nm) at 25°C. GLC/MS and LC/UV-DAD/MS analyses
were performed to investigate phototransformation products after a solid phase
extraction (SPE) step for analytes concentration. For each compound several by-products
have been identified and are the same ones under both irradiation conditions.
Because of the presence of the same chromophore (α, β-unsaturated group) absorbing UV radiations in both
hormones, the majority of chromatographic peaks correspond to by-products
formed according to identical mechanisms involving isomerization, enolization,
oxidation and hydration to lead to the generation of lumiketone,
cyclopentenone, spiro-hydration, oxidation and hydroxylation photoproducts.