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http://dx.doi.org/10.1038/nature08064

has been cited by the following article:

• TITLE: Influence of Saliva and Mucin on the Adhesion of Candida Oral Clinical Isolates

  AUTHORS: Catarina L. Seabra, Cláudia M. Botelho, Ana C. N. Oliveira, Mariana Henriques

  KEYWORDS: Candida, Artificial Saliva, Mucin, Oral Adhesion, Surface Properties

  JOURNAL NAME: Journal of Encapsulation and Adsorption Sciences, Vol.5 No.4, December 30, 2015

  ABSTRACT: Objectives: This research work intends to clarify the role of artificial saliva, in particularly the role of mucin, a salivary protein, on the surface properties and adhesion ability of Candida spp. oral clinical isolates to abiotic surfaces. Methods: Four oral clinical isolates of Candida spp. were used: two Candida albicans strains (AC; AM) and two Candida parapsilosis strains (AD; AM2). The strains were isolated from patients using oral prosthesis. The microorganisms were cultured in the absence or presence of mucin and artificial saliva, and their adhesion to an abiotic surface (coated with mucin and artificial saliva) was evaluated. Results: The presence of mucin per se onto the abiotic surface decreased the adhesion of all strains, although the combination of mucin with artificial saliva had reduced this effect. No direct correlation between adhesion and the surface free energies of adhesion of the microorganisms was found. Significance: Candida spp. were human commensal microorganisms that became pathogenic when the host immune defenses were compromised. Medical devices were colonized by Candida spp. particularly, oral prostheses, which might lead to the degradation of the prostheses and systemic infections. The salivary secretions that constantly cover the oral cavity influenced Candida spp. adhesion process. Therefore, it was important to understand the interactions between Candida spp., salivary proteins and the characteristic of oral prosthesis when developing materials for oral prostheses.