TITLE:
Modulation of a Specific Pattern of microRNAs, Including miR-29a, miR-30a and miR-34a, in Cultured Human Skin Fibroblasts, in Response to the Application of a Biofunctional Ingredient that Protects against Cellular Senescence in Vitro
AUTHORS:
Xianghong Yan, Catherine Serre, Laurine Bergeron, Ludivine Mur, Valère Busuttil, Jean-Marie Botto, Nouha Domloge
KEYWORDS:
microRNAs, qPCR Array, Skin Fibroblasts, Skin Senescence, Telomere, Extracellular Matrix, Sirtuin 1, Collagen
JOURNAL NAME:
Journal of Cosmetics, Dermatological Sciences and Applications,
Vol.5 No.4,
December
29,
2015
ABSTRACT: Skin aging is a process of structural and compositional remodeling that
can be manifested by wrinkling and sagging. Remarkably, the dermis plays a
dominant role in the aging process. Recent studies suggest that microRNAs are
implicated in the regulation of gene expression during aging. However, studies
about age-related microRNAs and how they modulate skin aging remain limited. In
the present work, a complex of hydrolyzed natural yeast proteins (Saccharomyces cerevisiae) and hydrolyzed
natural soya bean was developed and showed the ability to modulate the expression
of telomere-binding protein TRF2, which is a key factor for telomere protection
and to prevent cellular senescence in
vitro and DNA damage. The aim of the study was to identify microRNAs specifically
modulated after application of the ingredient complex to cultured fibroblasts,
and their possible involvement in remodeling of the human extracellular matrix
and fibroblast senescence. Consequently, human skin fibroblasts were cultured
and treated with 1% of the ingredient complex for 48 h before analyzing
microRNA modulation by RT-qPCR. The use of bioinformatics allowed us to predict
the target genes for modulated microRNAs. Results show that the ingredient
complex modulated a pattern of microRNAs including the down-regulation of
miR-29a-3p, miR-30a-5p and miR-34a-5p, which are associated with fibroblast
senescence and remodeling of the human dermal extracellular matrix. In
conclusion, our results indicate that miR-29a-3p, miR-30a-5p and miR-34a-5p
possibly represent key microRNAs that impact human fibroblast senescence and
remodeling of the dermal extracellular matrix.