TITLE:
The C161T Polymorphism in Peroxisome Proliferator-Activated Receptor ɣ2, but Not Pro12Ala, Is Associated with Diabetic Retinopathy in Type 2 Diabetes Mellitus in an Egyptian Population
AUTHORS:
Ragaa Abelkader Ramadan, Moyassar Ahmad Zaki, Rania Mohamed El Sharkawy, Lubna Mohamed Desouky, Marwa Ahmed Madkour, Karim Mahmoud Nabil Mohamed Kamel
KEYWORDS:
Type 2 Diabetes Mellitus, Diabetic Retinopathy, Polymorphism, PPAR០, Pro12Ala, C161T
JOURNAL NAME:
Journal of Diabetes Mellitus,
Vol.6 No.1,
December
25,
2015
ABSTRACT: Objectives: Diabetic retinopathy (DR) is one of the most common
microvascular complications of type 2 diabetes mellitus (T2DM). It is
multifactorial with the contribution of multiple genetic factors. We questioned
the association of polymorphisms in the peroxisome proliferator-activated
receptor ?2
(PPAR?2) gene (Pro12Ala
and C161T) with DR in an Egyptian population. Methods: This case control study
included one hundred healthy individuals and 252 T2DM among them 122 with DR
and 130 without DR. Genotyping was done by polymerase chain reaction
restriction fragment length polymorphism (PCR-RFLP). Results: The Pro12Ala
Ala allele was associated with decreased risk of DR with an odds ratio (OR) of 0.484,
95% confidence interval (CI) (0.254 - 0.920), and a p value = 0.024. The C161T
T allele was associated with increased risk of DR with OR = 2.593, 95% CI
(1.672 - 4.020), p 1c) in multivariate regression
analysis only C161T was associated with increased risk of DR with OR = 3.479,
95% CI (1.907 - 6.346), p 1c was higher in Pro/Pro genotype when
compared to those with Ala/Ala and Pro/Ala genotypes. Conclusion: We report
that T allele of C161T increased risk of DR in the studied population. Further
studies are warranted to investigate functional implications of polymorphisms
of the PPAR-? gene in DR development.