Rakels, J.L.L., Straathof, A.J.J. and Heijnen, J.J. (1993) A Simple Method to Determine the Enantiomeric Ratio in Enantioselective Biocatalysis. Enzyme and Microbial Technology, 15, 1051-1056.
http://dx.doi.org/10.1016/0141-0229(93)90053-5

has been cited by the following article:

• TITLE: Chemoenzymatic Synthesis of an Enantiomerically Enriched Bicyclic Carbocycle Using Candida parapsilosis ATCC 7330 Mediated Enantioselective Hydrolysis

  AUTHORS: Thangavelu Saravanan, Anju Chadha, Tarur Konikkaledom Dinesh, Namasivayam Palani, Sengottuvelan Balasubramanian

  KEYWORDS: Chemoenzymatic Synthesis, Enantioselective Hydrolysis, Candida parapsilosis ATCC 7330, Hydrolases, Bicyclic Carbocycle

  JOURNAL NAME: International Journal of Organic Chemistry, Vol.5 No.4, December 24, 2015

  ABSTRACT: Enantiomerically enriched (R)-1-(2-bromocycloalkenyl)-3-buten-1-ol and its derivatives were obtained via enantioselective hydrolysis [resolution] with good enantioselectivities (E = 31 to >500) using Candida parapsilosis ATCC 7330. The various reaction parameters were optimized for enantioselective hydrolysis to achieve high enantiomeric excess (ee) and conversions. Among the substrates tested, (RS)-1-(2-bromocyclohex-1-en-1-yl) but-3-yn-1-yl acetate was hydrolysed by the biocatalyst in 12 h to the corresponding (R)-alcohol in 49% conversion and >99 ee. The optically pure allylic alcohol thus obtained was used as a chiral starting material for the synthesis of an enantiomerically enriched bicyclic alcohol effectively establishing achemoenzymatic route.