TITLE:
Transgenic Mouse (Tg2576) Is an Ideal Model for the Biological Characterization of [18F]-FDDNP for Identifying Alzheimer’s Disease
AUTHORS:
Kang-Wei Chang, Shih-Ying Lee, Chia-Chieh Chen
KEYWORDS:
[18F]-FDDNP, Alzheimer’s Disease, Transgenic Mice, β-Amyloid Plaques
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.3 No.12,
December
18,
2015
ABSTRACT:
[18F]-FDDNP was synthesized and
characterized as a positron-emitting probe to identify Alzheimer’s disease
(AD) in transgenic mouse models (Tg2576 and dE9) expressing the AD pathology.
We observed in in vitro, in vivo, and ex vivo studies that [18F]-FDDNP accumulated specifically in the Ab-overexpressing brain regions and that this accumulation was
significantly reduced by co-incubation with non-radioactive FDDNP. In ex vivo
and in vivo studies of brain sections, the retention of radioactivity was more
specific in Tg2576 mice than in dE9 mice. Using in vitro, ex vivo, in vivo, and
ELISA analyses, we characterized the utility of [18F]-FDDNP in mapping b-amyloid in the Tg2576 mouse brain, to assess its potential
application in imaging strategies.