TITLE:
Isoenzyme Expression in Bean Seed Germination Treated with Thiamethoxam with and without Drought Stress
AUTHORS:
Arthur Blois Villela, Andreia da Silva Almeida, César Iván Suárez Castellanos, Cristiane Deuner, Vanessa Nogueira Soares, Thais Ongaratto de Camargo, Geri Eduardo Meneghello, Francisco Amaral Villela, Lilian Madruga de Tunes, Paulo Dejalma Zimmer
KEYWORDS:
Phaseolus vulgaris, Enzymes, Germination, Bioactivator
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.6 No.19,
December
11,
2015
ABSTRACT: The thiamethoxam acts as enhancer, allowing maximizing the expression of seed vigor. Isozymes
are products of gene expression, highly influenced by the environment, because genes that control
its expression are manifested in certain stages of development and in specific organs and tissues,
or even under the action of certain stimulus. This study evaluated the isoenzyme expression in
bean seedlings from seeds treated with thiamethoxam. Bean seeds were used, cultivar Pérola and
IAPAR Siriri, submitted or not to drought stress. Seeds were treated at doses of thiamethoxam: 0, 1,
2, 3, 4 and 5 mL·kg-1 of seed. Isozymes were extracted from seedlings collected five days after
sowing. We evaluated the expression of isoenzymes: Glutamate Oxalacetate transaminase (GOT),
acid phosphatase (FAC), peroxidase (PO), Esterase (EST), malate dehydrogenase (MDH) and glutamate
dehydrogenase (GTDH). Interpretation of the results was based on visual analysis of electrophoresis
gels, considering the presence/absence and the intensity of each electrophoretic band.
Bean seeds treated with thiamethoxam with doses up 3 mL·kg-1 of seed are higher germination
untreated, even when submitted to drought stress. The expression of isozymes GOT, FAC, PO, EST,
MDH and GTDH is not affected significantly in bean seedlings of Pérola and IPR Siriri under different
doses of thiamethoxam with and without drought stress.