TITLE:
Effects of Media Composition and Auxins on Adventitious Rooting of Bienertia sinuspersici Cuttings
AUTHORS:
Jennifer Anne Northmore, Marie Leung, Simon Dich Xung Chuong
KEYWORDS:
Bienertia sinuspersici, Single-Cell C4 Photosynthesis, MS Media, Auxins, Adventitious Roots
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.6 No.10,
October
12,
2015
ABSTRACT: An efficient in vitro method for rapid vegetative propagation of Bienertia sinuspersici, one of four
terrestrial species of family Chenopodiaceae capable of performing C4 photosynthesis within a
single cell, was developed. Cuttings of B. sinuspersici were used to examine the effects of Murashige
and Skoog (MS) media strength and auxins on adventitious root formation. Half-strength MS medium
was determined to be ideal for adventitious root formation in Bienertia cuttings. Although
cuttings cultured in medium containing 5.0 mg/L α-naphthalene acetic acid (NAA) promoted the
highest number of adventitious roots, cuttings cultured in medium supplemented with 1.0 mg/L
indole-3-butyric acid (IBA) produced the longest adventitious roots and had the highest survival
rate upon transplanting to soil. Histological analysis revealed variations in the root anatomy generated
by the various auxins which may affect adventitious root formation and subsequent establishment
of cuttings in soil. Overall, the established procedure provides a simple and cost-effective
means for the rapid propagation of the single-cell C4 species B. sinuspersici.