Fujii, Y., Tanaka, R., Miyake, H., Tamaru, Y., Ueda, M. and Shibata, T. (2013) Evaluation for Antioxidative Properties of Phlorotannins Isolated from the Brown Alga Eisenia bicyclis, by the H-ORAC Method. Food and Nutrition Sciences, 4, 78-82.
http://dx.doi.org/10.4236/fns.2013.48A010

has been cited by the following article:

• TITLE: Analysis on Composition and Antioxidative Properties of Phlorotannins Isolated from Japanese Eisenia and Ecklonia Species

  AUTHORS: Toshiyuki Shibata, Kohki Nagayama, Shingo Sugiura, Saki Makino, Mitsuyoshi Ueda, Yutaka Tamaru

  KEYWORDS: Eisenia Species, Ecklonia Species, Cultured E. kurome, H-ORAC, Phlorotannins, Radical Scavenging Activities

  JOURNAL NAME: American Journal of Plant Sciences, Vol.6 No.15, September 30, 2015

  ABSTRACT: The composition and antioxidative properties of crude phlorotannins isolated from Japanese Eisenia and Ecklonia species including cultured E. kurome were analyzed. As a result of liquid chromatography mass spectrometry analysis, it was suggested that the phlorotannins were composed of compounds containing 3 - 7 phloroglucinol units for Eisenia species and 3 - 8 phloroglucinol units for Ecklonia species. The antioxidant properties of phlorotannins were evaluated using the hydrophilic oxygen radical absorbance capacity (H-ORAC) assay, the measurements of superoxide anion radical scavenging activity (SOSA) with electron spin resonance method, and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and reducing power assay. It was clarified that the phlorotannins contained H-ORAC values of 8.9 × 103 - 1.0 × 104 μmol-Trolox equivalents (TE)/g, SOSA of 1.5 × 104 - 2.9 × 104 superoxide dismutase units/g, DPPH radical scavenging activity of 4.7 × 103 - 5.5 × 103 μmol-TE/g, and reducing power of 6.5 × 102 - 7.0 × 102 mg-ascorbic acid equivalents/g, and had excellent antioxidative properties.