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http://dx.doi.org/10.1021/ac900095h

has been cited by the following article:

• TITLE: Online Effective Identification of Glycopeptide Using Liquid Chromatography Combined with Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FTICR-MS)*

  AUTHORS: Ling Xin, Li Hou, Yue Zhou, Huiping Wang, Heming Yu

  KEYWORDS: Glycopeptide, LC-ESI, FTICR-MS

  JOURNAL NAME: Advances in Bioscience and Biotechnology, Vol.6 No.9, September 24, 2015

  ABSTRACT: The detailed glycan structural analysis of glycoprotein is amenable to glycopeptide enrichment. Here, we develop a simple, effective and economical approach to enrich glycopeptides from proteolytically digested peptide mixtures by chromatographic column packed with graphite carbon and activated charcoal (G/A-column). Glycopeptide from ovalbumin was efficiently enriched by homemade G/A-column using liquid chromatography and the structure of glycopeptide was obtained by tandem mass spectrometry using Fourier transform ion cyclotron resonance mass spectrometry. The results in this study demonstrate that G/A-column can be used to enrich N-glycolpeptides and be benefit for online identification of glycopeptide using LC-MS.