TITLE:
Chitinolytic Assay and Identification of Bacteria Isolated from Shrimp Waste Based on 16S rDNA Sequences
AUTHORS:
Imanda N. Setia, Suharjono
KEYWORDS:
Chitinolytic Bacteria, Chitin, Shrimp, Shrimp Waste
JOURNAL NAME:
Advances in Microbiology,
Vol.5 No.7,
July
22,
2015
ABSTRACT: Shrimp waste contains 20% - 60% chitin and possible to be source of
chitinolytic bacteria. Chitinolytic bacteria are capable of hydrolyzing of chitin
progressively to produce N-acetylglucosamine monomer which can be used to
overcome the shrimp waste. The objectives of this research were to identify species of bacteria
with high activity of chitin degradation in shrimp waste and to analyze their potency
as chitin degradation agent. The research consists of screening of chitinolytic
bacteria based on chitinolytic index, activity assay of chitinase using colorimetric method, and molecular identification of bacteria based on 16S
rDNA sequences. Two of eighteen isolates of chitinolytic bacteria
(PBK 2 and SA 1.2 isolates) showed the highest chitinolytic index, which were
2.069 and 2.084, whereas chitinase activity was 0.213 and 0.219 U/ml
respectively. Based on 16S rDNA sequences, isolate of PBK 2 was identified as Acinetobacter johnsonii 3-1, whereas SA 1.2
was identified as Bacillus amyloliquefaciens GR53 with 99.78%
similarity.