TITLE:
Diagnostic and Analysis of Human Sperm Characteristics Using Fourier Transform Infrared Spectroscopy
AUTHORS:
Amir Abramovich, Alexander Shulzinger
KEYWORDS:
Human Sperm, FTIR, Spectroscopic Measurements
JOURNAL NAME:
Open Journal of Urology,
Vol.5 No.6,
June
30,
2015
ABSTRACT: A spectroscopic method for human sperm
evaluation and characterization using Fourier Transform Infra Red (FTIR) is
presented. The high sensitivity of FTIR to changes in chemical structure and
arrangement of molecules and proteins makes it a powerful diagnostic tool. Our
experimental results show that a simple MIR (400 cm-1- 4000 cm-1) transmission
spectrum of a human sperm is very fast and can be used to determine the level
of structure, compare to conventional LAB tests. No sample preparations are
required, the semen has to be put on a special ZnSe substrate and inserted into
the measurement compartment of the FTIR. Furthermore, this method can
distinguish between immature sperm cell to white blood cell which by using a
microscope is difficult and re-quires experience.