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http://dx.doi.org/10.1016/0147-5975(89)90009-1

has been cited by the following article:

• TITLE: Expression of T4HR1, a 1,3,6,8-Tetrahydroxynaphthalene Reductase Gene Involved in Melanin Biosynthesis, Is Enhanced by Near-Ultraviolet Irradiation in Bipolaris oryzae

  AUTHORS: Nozomi Tanaka, Yohei Haruki, Makoto Ueno, Sakae Arase, Junichi Kihara

  KEYWORDS: Bipolaris oryzae, Gene Expression, Melanin Biosynthesis, Near-Ultraviolet, 1, 3, 6, 8-Tetrahydroxynaphtalene Reductase

  JOURNAL NAME: Advances in Microbiology, Vol.5 No.3, March 6, 2015

  ABSTRACT: Bipolaris oryzae is the causal agent of brown spot disease in rice and produces the dark pigment melanin. We isolated and characterized T4HR1 gene encoding 1,3,6,8-tetrahydroxynaphthalene (1,3,6,8-THN) reductase, which converted 1,3,6,8-THN to scytalone in the melanin biosynthesis from B. oryzae. A sequence analysis showed that the T4HR1 gene encoded a putative protein of 268 amino acids showing 50% - 99% sequence identity to other fungal 1,3,6,8-THN reductases. Targeted disruption of the T4HR1 gene showed a different phenotype of mycelial color due to an accumulation of shunt products compared to those of wild-type on PDA plates using tricyclazole as a melanin biosynthesis inhibitor. A quantitative real-time PCR analysis showed that the expression of T4HR1 transcripts was enhanced by near-ultraviolet (NUV) irradiation and regulated by transcriptional factor BMR1, similar to three other melanin biosynthesis genes (polyketide synthase gene [PKS1], scytalone dehydratase gene [SCD1], and 1,3,8-THN reductase gene [THR1]) in the melanin biosynthesis of B. oryzae. These results suggested that common transcriptional mechanisms could regulate the enhanced gene expression of these melanin biosynthesis genes by NUV irradiation in B. oryzae.