TITLE:
Loop-Mediated Isothermal Amplification (LAMP) for the Detection of Listeria monocytogenes and Major Pathogenic Serotypes
AUTHORS:
Ana Paula Rocha da Costa, Mariana de Lira Nunes, Carina Lucena Mendes-Marques, Alzira Maria Paiva de Almeida, Nilma Cintra Leal
KEYWORDS:
Listeria monocytogenes, Diagnosis, Serotypes, Identification, LAMP
JOURNAL NAME:
American Journal of Analytical Chemistry,
Vol.5 No.16,
November
25,
2014
ABSTRACT: Rapid
identification and characterization of Listeria
monocytogenes are required for the food industry, epidemiological studies,
and disease prevention and control. However, typing procedures are labor-intensive
and time-consuming, and they require technical expertise, a panel of sera and
reference culture strains or sophisticated and expensive equipment. To improve
upon traditional diagnostic methods for L.
monocytogenes we developed and evaluated an efficient procedure for the
specific identification of L.
monocytogenes and the major pathogenic serotypes of the species based on
loop-mediated isothermal amplification (LAMP). Four individual reactions were designed
using primers targeting any L.
monocytogenes serotypes (LAMP-AS) and the 1/2a (LAMP-1/2a), 1/2b (LAMP-1/2b), and 4b (LAMP-4b) serotypes. The procedure distinguished L. monocytogenes from closely
genetically related species and the targeted serotypes. Cross-reactivity with a
few rare serotypes isolated from food or clinical samples did not impair the
usefulness of the procedure. Thus, our approach constitutes a fast, easy and
low-cost alternative for L. monocytogenes diagnosis and serotyping and may be useful for surveillance and epidemiological
investigation programs.