TITLE:
Phylogenetic Analysis of Baculovirus Isolates from Diseased Insects in Southern Vietnam
AUTHORS:
Nguyen Thi Phuong Thao, Nguyen Thi Thuy, Nguyen Khac Duy, Doan Chinh Chung, Do Minh Si, Le Thanh Long
KEYWORDS:
Baculovirus, Helicoverpa armigera, Helicoverpa zea, Lef-8 Gene, Phylogeny, Spodoptera litura
JOURNAL NAME:
Open Journal of Genetics,
Vol.4 No.5,
September
2,
2014
ABSTRACT:
The aim of this study was
to investigate the molecular identification and assess the genetic relationship
of baculovirus isolated from Southern Vietnam. The diseased insect samples were
collected from the different fields. The partial sequence of 450 base pairs of
lef-8 gene was amplified and sequenced to assess the genetic variations of
baculovirus isolates specific for Spodoptera
litura, Helicoverpa zea, and Helicoverpa armigera. The sequences
alignment demonstrated that Helicoverpa
zea specific isolates exhibited six single nucleotide polymorphic sites.
Whereas, twenty five single polymorphic sites were found in Spodoptera litura specific isolates.
Thus, Spodoptera litura specific isolates
were higher polymorphic than Helicoverpa
zea specific isolates. The genetic distance analyses showed that the
distance between Vietnamese baculovirus isolates and Group II Alphabaculovirus
isolates was lower than other Baculovirus groups. The phylogeny of Vietnamese isolates in relation to other baculovirus isolates
was also determined using partial sequences of lef-8 gene. The phylogenetic
tree placed all Vietnamese isolates in Group II Alphabaculovirus, where seven
Vietnamese Helicoverpa zea specific
isolates were most closely related to Helicoverpa
zea SNPV, fourteen Vietnamese Spodoptera litura specific isolates were
located with Spodoptera litura NPV-G2
in one clade and a Vietnamese Helicoverpa
armigera isolate was appeared to be closely related to Helicoverpa armigera SNPV-NNg1, Helicoverpa
armigera NPV-C1, Helicoverpa armigera NPV-G4.