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has been cited by the following article:

• TITLE: Isolation and Characterization of 26 Microsatellite Loci for the Shortfin Silverside Fish Chirostoma humboldtianum Valenciennes1835 (Atherinopsidae: Menidiinae) Derived from Next Generation Sequencing and Their Cross-Amplification in Central Mexican Atherinopsids

  AUTHORS: Barriga-Sosa Irene de los Angeles, Rosa María García-Martínez, Jhoana Díaz-Larrea, Oscar Adrián Lozano Garza, Francisco Javier García-De-León

  KEYWORDS: Endemic, Polymorphism, Villa Victoria, Poblana alchichica, C. promelas, C. riojai

  JOURNAL NAME: Open Journal of Genetics, Vol.4 No.5, August 18, 2014

  ABSTRACT: The endemic silverside fish C. humboldtianum is of great ichtyologycal, economical and cultural relevance in central Mexico and it has been suggested that it is among a group of other “peces blancos”, the most ancestral species. Here we characterized a set of 26 microsatellite loci from the species in order to further assess population and phylogeographic issues that aid in evaluating their highly impacted populations. From 58 primer pairs tested on specimens from Villa Victoria dam (Rio Balsas Basin), 26 loci positively amplified on the species and cross-primed on specimens of the closely related and threatened Poblana alchichica, C. promelas and C. riojai. Loci resolved medium and high genetic variation (mean NA = 14.3, SD = 9.38; mean HO & HE = 0.47, SD = 0.32 and 0.58, SD = 0.32, respectively) and it is expected that these polymorphic loci are also useful in studing threatened atherinopsid species.