TITLE:
Dodecylamine Derivative of Hydroxocobalamin Acts as a Potent Inhibitor of Cobalamin-Dependent Methionine Synthase in Mammalian Cultured COS-7 Cells
AUTHORS:
Tomohiro Bito, Mariko Yasui, Toshio Iwaki, Yukinori Yabuta, Tsuyoshi Ichiyanagi, Ryoichi Yamaji, Yoshihisa Nakano, Hiroshi Inui, Fumio Watanabe
KEYWORDS:
Dodecylamine Derivative, Cobalamin, COS-7 Cell, Enzyme Inhibitor, Hydroxocobalamin
JOURNAL NAME:
Food and Nutrition Sciences,
Vol.5 No.14,
August
6,
2014
ABSTRACT: We
evaluated whether the dodecylamine derivative of hydroxocobalamin acts as a
potent inhibitor of cobalamin-dependent enzymes in an African green monkey
kidney cell, COS-7. When the dodecylamine derivative (1.0 μmol/L) did not show
any cytotoxicity in the cultured cells, the derivative could not affect
methylmalonyl-CoA mutase (holo-enzyme) activity, but significantly inhibit
methionine synthase (holo-enzyme) activity in the cell homogenates of COS-7
grown in 1.0 μmol/L hydroxocobalamin-supplemented medium. An immunoblot
analysis indicated that the dodecylamine derivative could not decrease the
protein level of methionine synthase, but significantly inhibit the enzyme
activity.