T. Noro, Y. Oda, T. Miyase, A. Ueno and S. Fukushima, “Inhibitors of Xanthine Oxidase from the Flowers and Buds of Daphne Genkwa,” Chemical Pharmaceutical Bulletin (Tokyo), Vol. 31, 1983, pp. 3984-3987.

has been cited by the following article:

• TITLE: A New Diterpene Extracted from Daphne Mucronata, Effects on Human K562 and CCRF-CEM Cell Lines

  AUTHORS: Mehdi Hedayati, Razieh Yazdanparast, Marjan Zarif Yeganeh, Laleh Hoghooghi Rad, Fereidoun Azizi

  KEYWORDS: TNF-α, Leukemia, Daphne Mucronata, K562, CCRF-CEM

  JOURNAL NAME: Journal of Cancer Therapy, Vol.2 No.1, March 15, 2011

  ABSTRACT: Background: Daphne Mucronata extract has a decreasing effect on the size of breast adenocarcinoma in rats. So in this study, the effect of Daphne Mucronata purified diterpene were investigated on co culture of human monocytes and two human leukemia cell lines (K562, CCRF-CEM). Materials and Methods: Each cell line mono-layer culture, in log phase growth, was treated with 10 to 160 μL of the extract (1 g/ml leave powder) and purified compound (0.94 nM). For a comparative study, Taxol (5 to 40 μM) was used in the presence and absence of LPS. Human monocytes were isolated by adhesion method. TNF-α in cultured media were measured by sensitive biotin-streptoavidin ELISA method. Results: Fifty percent of growth inhibition was shown by 160 μL (1:100 dilution, 0.5 g of the powdered leaves/ml) of the extract and 0.94 nM of the purified component, and there was more inhibition in K562 cells (P