TITLE:
Hormonal Requirements Trigger Different Organogenic Pathways on Tomato Nodal Explants
AUTHORS:
Salem Jehan, A. M. Hassanein
KEYWORDS:
Growth Regulators; Nodal Segment; Organogenesis; Tissue Culture; Tomato
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.4 No.11,
November
13,
2013
ABSTRACT:
In this work,
nodal-disk segments (4-6 mm in diameter × 5-6 mm in length) were
obtained from established shoot culture, resulted from disinfected tomato
seedlings, and they were suitable to induce different organogenic pathway under
the influence of specific hormonal treatment. Application of BAP (1-2.5 mg/l)
alone or in combination of 0.5 mg/l NAA resulted in induction of shoot formation.
Somatic embryogenesis was rarely appeared (6%) when relatively low
concentration of BAP (1.5 mg/l) with low concentration of IAA (0.5 mg/l IAA)
was applied. Root induction was triggered when nodal explants or shoot cuttings
were cultured on MS medium with (1 mg/l IAA, IBA or NAA) or without auxins, but
the best result was obtained when 1 mg/l IAA was used. Application of 0.5 mg/l
NAA stimulated callus formation but the best result was obtained when the three
different phytohormoes were used (0.5 mg/l 2,4-D + 1 mg/l NAA + 0.5 mg/l BAP).
These results indicated that nodal segments, as described in this protocol, can
be used as alternative to other types of explants such as cotyledon, hypocotyl
and leaf explants.