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Conference on Environmental Pollution and Public Health (CEPPH 2010 E-BOOK)

Wuhan,China,China,9.10-9.12,2010

ISBN: 978-1-935068-16-7 Scientific Research Publishing, USA

E-Book 1502pp Pub. Date: October 2010

Category: Engineering

Price: $80

Title: The Effect of Uranium Stress to Yeast Cell Antioxidant Enzymes Activities under Acute Conditions
Source: Conference on Environmental Pollution and Public Health (CEPPH 2010 E-BOOK) (pp 338-341)
Author(s): Dong Zhang, Institute of Nuclear Physics and Chemistry, China Academy of Engineering Physics, Mianyang,Sichuan 621900, China
Ming-xue Liu, Life Science and Engineering College, Southwest University of Science and Technology, Mianyang, Sichuan, 621010, China/National Defense Key Discipline Laboratory of the Nuclear Waste and Environmental Safety of the Commission of Science, Technology and Ind
Hou-jun Kang, Institute of Nuclear Physics and Chemistry, China Academy of Engineering Physics, Mianyang,Sichuan 621900, China
Wei Zhang, National Defense Key Discipline Laboratory of the Nuclear Waste and Environmental Safety of the Commission of Science, Technology and Industry for National Defense, Southwest University of Science and Technology, Mianyang, Sichuan 621010, China
Ye Li, Institute of Nuclear Physics and Chemistry, China Academy of Engineering Physics, Mianyang,Sichuan 621900, China
Qing-bi Gou, Life Science and Engineering College, Southwest University of Science and Technology, Mianyang, Sichuan, 621010, China
Fa-qin Dong, National Defense Key Discipline Laboratory of the Nuclear Waste and Environmental Safety of the Commission of Science, Technology and Industry for National Defense, Southwest University of Science and Technology, Mianyang, Sichuan 621010, China
Abstract: The malonaldehyde(MDA) and three antioxidant enzymes activity changes of yeast (Saccharomyces cerevisiae) have been investigated under uranium acute stress to explore the cell tolerance mechanism to uranium stress in this research. The results showed that yeast cell may be affected by oxidative stress and lead to oxidative damage of membrane lipid as a results of the increase of MDA contents under low uranium concentration short time stress conditions, then the cell would be induced to increase the antioxidant enzyme activity to scavenge the oxidative stress as a result of decrease of MDA contents with the culture time prolonging. While the high concentration uranium stress may inhibit the antioxidant enzyme activity and lead to irreversible oxidative damage as a result of invariable increase of MDA contents. We suggested that yeast cell tolerance mechanism to uranium under acute stress conditions were: 1) the low uranium stress may induce cell to increase the antioxidant enzyme activity to scavenge oxidative damage and 2) the high concentration uranium may form precipitate or crystal with the cell wall or cell secretion substance outside of the cell to avoid acute lethal action to yeast cell under acute stress conditions but it yet will lead to irreversible oxidative damage.
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