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Title: |
PGPR (Plant Growth-Promoting Rhizobacteria) in Mulberry Rhizosphere with the Potential of Soil Fertilizer Pollution Control |
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Source: |
Conference on Environmental Pollution and Public Health (CEPPH 2010 E-BOOK)
(pp 306-309)
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Author(s): |
Fan Wu, College of Forestry, Shandong Agricultural University/Industrial Crops Institute of Hubei Academy of Agricultural Sciences
Zhen Song, College of Life Science, Shandong Agricultural University
Nan Zhang, College of Life Science, Shandong Agricultural University
Hui Guo, College of Life Science, Shandong Agricultural University
Benfeng Zhang, College of Forestry, Shandong Agricultural University,
Nianquan Qiu, College of Forestry, Shandong Agricultural University,
Xunli Liu, College of Forestry, Shandong Agricultural University, |
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Abstract: |
PGPR in mulberry rhizosphere were investigated in order to provide a theoretical basis for improving the soil fertility by biological effect and reducing the chemical fertilizer pollution. The three-point sampling methods were carried out in mulberry soil with different fertilities in Silkworm and Mulberry Scientific Research Station of Shandong Agricultural University, where the mulberry species Husang 32 were planted. Some PGPR were isolated from the samples and the repetitive-element PCR (Rep-PCR) genomic fingerprinting and biological activities were analyzed. The strains with positive biological activities were identified by the analysis of 16S rDNA sequence. The results showed that, based on the analysis of Rep-PCR, 18 kinds of azotobacteria, 18 kinds of phosphor-bacteria and 23 kinds of silicate bacteria were obtained from the samples, respectively. PA19 and PA2 have the highest nitrogenase activities among the azotobacteria. PYP2, PYP4, PYP6, PYP3 have higher organic phosphate solubilizing activities than that of others. FK14, PWP4 have higher inorganic phosphate solubilizing activities than that of others. The potassium-releasing activity of FK2 and FK3 were higher than that of other silicate bacteria. According to identification, 10 isolates with high biological activities were identified as follows: PA19 belonged to genus Mesorhizobium, PA2, PYP2, PYP6, PYP3 and FK2 belonged to genus Pseudomonas, PWP4 and FK14 belonged to genus Rhizobium, FK3 belonged to genus Sinorhizobium, and PYP4 belonged to genus Inquilinum.
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