Antimicrobial Activity of Terminalia catappa Extracts Against Some Pathogenic Microbial Strains

Abul Manzur, Arifuddin Raju, Shahedur Rahman

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DOI: 10.4236/pp.2011.24038                

Abstract

The methanol, acetone and N, N-dimethylformamide extracts of Terminalia catappa L. leaf were evaluated for antibacterial and antifungal activity. Piperacillin and gentamicin were used as standards for antibacterial assay, while nystatin and flucanazole were used as standards for antifungal assay. 91 clinically important strains were used for the study which were both clinical isolates as well as identified strains. The antimicrobial activity of all the extracts was determined by agar disc diffusion method. The antibacterial activity was more pronounced against bacteria than fungal strains. The Gram positive bacteria were more susceptible than Gram negative bacteria. The methanol extract showed best antibacterial activity. T. catappa leaf extracts showed better antibacterial activity than commercially used antibiotics.

Keywords

Terminalia Catappa, Antibacterial Activity, Antifungal Activity, Clinical Strains, Organic Solvents

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	G. M. Cragg and D. J. Newman, In: M. M. Iwu and J. C. Wootton, Eds., “Drugs from nature: Past achievements, future prospects, Ethnomedicine and Drug discovery”, Elsevier Science, Amsterdam, 2002, pp. 23-37.
[2]	A. A. Allero and A. J. Afolayan, “Antimicrobial activity of Solanum tomentosum,” African Journal of Biotechnology, Vol. 5, 2006, pp. 369-372.
[3]	B. Abu-Shanab, G. Adwan, D. Abu-Safiya, N. Jarrar and K. Adwan, “Antibacterial activities of some plant extracts utilized in popular medicine in Palestine”, Turkish Journal of Biology, Vol. 28, 2004, pp. 99-102.
[4]	M. R. F. De Lima, J. De Souza Luna, A. F. Dos Santos, M. C. C. De Andrade, A. E. G. Sant Ana, J. P. Genet, B. Marquez, L. Neuville and N. Moreau, “Antibacterial activity of some Brazilian medicinal plants”, Journal of Ethnopharmacology, Vol. 105, 2005, pp. 137-147.
[5]	J. Parekh, N. Karathia and S. Chanda, “Screening of some traditionally used medicinal plants for potential antibacterial activity”, Indian Journal of Pharmaceutical Science, Vol. 68, 2006, pp. 832-834.
[6]	J. Parekh and S. Chanda, “Antibacterial and phytochemical studies on twelve species of Indian medicinal plants”, African Journal of Biomedical Research, Vol. 10, 2007a, pp. 175-181.
[7]	S. Dash, L. K. Nath, S. Bhise and B. Nihar, “Antioxidant and antimicrobial activities of Heracleum nepalense D Don root”, Tropical Journal of Pharmaceutical Research, Vol. 4, 2005, pp. 341-347.
[8]	R. Nair, T. Kalariya and S. Chanda, “Antibacterial activity of some selected Indian medicinal flora”, Turkish Journal of Biology, Vol. 69, 2005, pp. 41-47.
[9]	J. Parekh, R. Nair and S. Chanda, “Preliminary screening of some folklore plants from western India for potential antimicrobial activity”, Indian Journal of Pharmacology, Vol. 37, 2005, pp. 408-409.
[10]	R. Nair and S. Chanda, “Antibacterial activity of some medicinal plants against some medically important bacterial strains”, Indian Journal of Pharmacology, Vol. 38, 2006, pp. 142-144.
[11]	M. B. Tadhani and R. Subhash, “In vitro antimicrobial activity of Stevia rebaudiana Bertoni leaves”, Tropical Journal of Pharmaceutical Research, Vol. 5, 2006, pp. 557-560.
[12]	S. Mandal, M. D. Mandal and N. Pal, “Antibacterial potential of Azadirechta indica seed and Bacopa monniera leaf extracts against multidrug resistant Salmonella enterica serovar Typhi isolates”, Archives of Medical Science, Vol. 3, 2007, pp. 14-18.
[13]	R. Nair and S. Chanda, “In-vitro antimicrobial activity of Psidium guajava L. leaf extracts against clinically important pathogenic microbial strains”, Brazilian Journal of Microbiology, Vol. 38, 2007, pp. 452-458
[14]	N. Y. Chiu and K.H. Chang, “The Illustrated Medicinal Plants of Taiwan”, SMC Publishing, Inc., Taipei, Vol. 1, 1986, pp. 129.
[15]	C.C. Lin, Y. L. Chen, J. M. Lin and T. Ujiie, “Evaluation of the antioxidant and hepatoprotective activity of Terminalia catappa”, American Journal of Chinese Medicine, Vol. 25, 1997, pp. 153–161.
[16]	A. N. Nagappa, P. A. Thakurdesai, N. Venkat Rao and J. Singh, “Antidiabetic activity of Terminalia catappa Linn fruits”, Journal of Ethnopharmacology, Vol. 88, 2003, pp. 45–50.
[17]	Y. M. Fan, L. Z. Xu, J. Gao, Y. Wang, X. H. Tang, X. N. Zhao and Z. X. Zhang, “Phytochemical and anti-inflammatory studies on Terminalia catappa”, Fitoterapia, Vol. 75, 2004, pp. 253–260.
[18]	Y. F. Zhai, J. Yao, Y. M. Fan, L. Z. Xu, J. Gao and X. N. Zhao, “Inhibitory effects of LR-98 on proliferation of hepatocarcinoma cells”, Journal of Nanjing University of Natural Science, Vol. 37, 2001, pp. 213–217.
[19]	L. Z. Xu, J. Gao, L. Zhu, M. Xu, S. Y. Lu, X. N. Zhao and Z. X. Zhang, “Protective effects of LR-98 on hepatotoxicity induced by carbon tetrachloride and D-galactosamine in mice”, Journal of Nanjing University of Natural Science, Vol. 36, 2000, pp. 197–201.
[20]	A. W. Bauer, W. M. M. Kirby, J. C. Sherries and M. Truck, “Antibiotic susceptibility testing by a standardized single disk method”, American Journal of Clinical Pathology, Vol. 45, 1966, pp. 426-493.
[21]	J. Parekh and S. Chanda, “In vitro antimicrobial activity of Trapa natans L fruit rind extracted in different solvents”, African Journal of Biotechnology, Vol. 6, 2007b, pp. 766-770.
[22]	M. J. Pelczar, E. C. S. Chan and N. R. Krieg, “Microbiology concepts and applications”, Mc Graw-Hill Inc, New York, 1993, pp. 967.
[23]	M J. Martinez, J. Betancourt, N. Alanso-Gonzalea and A. Jauregui, “Screening of some Cuban medicinal plants for antimicrobial activity”, Journal of Ethnopharmacology, Vol. 52, 1996, pp. 171-174.
[24]	R. F. Service, “Antibiotics that resist resistance”, Science, Vol. 270, 1995, pp. 724-727.
[25]	I. Ahmad, Z. Mehmood and F. Mohammad, “Screening of some Indian medicinal plants for their antimicrobial properties”, Journal of Ethnopharmacology, Vol. 62, 1998, pp. 183-193.
[26]	G. A. Cordell, “Biodiversity and drug discovery a symbiotic relationship”, Phytochemistry, Vol. 55, 2000, pp. 463-480.
[27]	B. Dulger and A. Gonuz, “Antibacterial activity of the endemic Hypericum kazdaghensis”, Fitoterapia, Vol. 76, 2005, pp. 237-239.
[28]	L. Stuart, “Infectious diseases and rise of antibiotic resistance”, Pharmaceutical News, Vol. 3, 1996, pp. 21.
[29]	H. Babayi, I. Kolo, J. I. Okojun and U. J. J. Ijah, “The antimicrobial activities of methanolic extracts of Eucalyptus camaldulensis and Terminalia catappa against some pathogenic microorganisms”, Biokemistri, Vol. 16, 2004, pp. 106-111.
[30]	J. E. Kelmanson, A. K. Jager and J. Van staden, “Zulu medicinal plants with antimicrobial activity”, Journal of Ethnopharmacology, Vol. 69, 2000, pp. 241-246.
[31]	J. Parekh and S. Chanda, “Screening of some India medicinal plants for antibacterial activity”, Indian Journal of Pharmaceutical Science, Vol. 68, 2006, pp. 835-838.
[32]	V. Prashanth Kumar, S. C. Neelam, H. Padh and M. Rajni, “Search for antibacterial and antifungal agents from selected Indian medicinal plants”, Journal of Ethnopharmacology, Vol. 107, 2006, pp. 182-188.
[33]	J. Yao and R. Moellering, “Antibacterial agents”, In: Manual of Clinical Microbiology, P. Murray, E. Baron, M. Pfaller, F. Tenover and R. Yolken, Eds, ASM, Washington DC, 1995, pp. 1281-1290.
[34]	I. Bhattacharjee, A. Ghosh and G. Chandra, “Antimicrobial activity of the essential oil of Cestrum diurnum L. Solanales: Solanaceae”, African Journal of Biotechnology, Vol. 4, 2005, pp. 371-374.
[35]	F. Scazzocchio, M. F. Cometa, L. Tomassina and M. Palmery, “Antimicrobial activity of Hydrastis canadensis extract and it’s major isolated alkaloids”, Planta Medica, Vol. 67, 2001, pp. 561-563.