Muscle and adipose tissue biopsy in older adults with type 2 diabetes

Yi Wang, David Simar, Kylie Anderberg, Yorgi Mavros, Shelley Kay, Renru Zhao, Bernhard T. Baune, Anthony O’Sullivan, Nalin Singh, Maria Fiatarone Singh

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DOI: 10.4236/jdm.2011.13005                

Abstract

We describe a method for obtaining skeletal muscle and subcutaneous adipose tissue in a single percutaneous needle biopsy procedure. Biopsies were performed in a double-blind randomized, sham-exercise controlled clinical trial of power training in 103 older adults with type 2 diabetes. In total 110 biopsies (73.3%) were performed in the first 50 participants at 3 time points. Muscle samples were obtained in 102/110 biopsies (92.7%); adipose samples were obtained in 72/110 biopsies (65.5%); both tissue types were obtained in 65/110 biopsies (59.1%). The average weight of muscle samples was 131±92 mg and the median weight of adipose samples was 51 mg (range 3 to 412 mg). No adverse effects of biopsies were reported other than mild transient pain or light-headedness. This is one of the first reports of both adipose and muscle tissue obtained during the same biopsy, and the first to describe in detail sampling technique, yield and safety.

Keywords

Biopsy; Skeletal Muscle; Subcutaneous Adipose Tissue; Type 2 Diabetes

Conflicts of Interest

The authors declare no conflicts of interest.

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