Studies on Antibiotic Sensitivity Pattern of Pseudomonas aeruginosa Isolated from Hospitalized Patients

Abstract

This study was performed to evaluate the antibiotic sensitivity pattern and determination of MIC (Minimum inhibitory concentration) and MBC (Minimum bactericidal concentration) value of Pseudomonas aeruginosa. The samples were collected from hospitalized and outdoor patients of Chittagong Maa-O-Shisu General hospital (October, 2010 to April, 2011). From the sample (blood, pus and urine) the isolate was identified on the basis of their morphological, cultural and biochemical characteristics. Antibiotic sensitivity pattern of the isolates was then observed (Disc diffusion method). It was found that Ciprofloxacin, Azithromycin and Chloramphenicol showed the highest percentages of sensitivity (above 60%) so they were the most effective drug for the treatment of Pseudomonas aeruginosa infection, where Vancomycin, Amphicillin and Amoxicillin showed the lowest percentages of sensitivity as well as less effective drug. Minimum Inhibitory Concentration (MIC) (Turbidimetric Method) values were determined against six antibiotics (Ciprofloxacin, Azithromycin, Kanamycin, Erythromycin, Chloramphenicol and Gentamycin). The MIC of Ciprofloxacin and Azithromycin for the majority of clinical isolates was 2 to >64 μg/ml. Like that the MIC of Gentamycin, Kanamycin and Chloramphenicol for the clinical isolates was 4 to >64 μg/ml. MIC value of Erythromycin was between 8 to >64 μg. The Minimum bactericidal concentration (MBC) for all these six drugs were also done and the result was between 2 to >64 μg/ml.

Share and Cite:

Chowdhury, S. , Naher, J. , Mamun, A. , Khan, R. , Ferdous, J. and Sultana, S. (2014) Studies on Antibiotic Sensitivity Pattern of Pseudomonas aeruginosa Isolated from Hospitalized Patients. Open Access Library Journal, 1, 1-9. doi: 10.4236/oalib.1100911.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] Buchanon, U.R.E. and Gibbons, N.E. (1974) Bergey’s Manual of Determinative Bacteriology. 8th Edition, The Williams and Wilkins Company, Baltomore.
[2] Brooks, G.F., Butel, J.S. and Moore, S.A. (2004) Jawetz, Melnick and Adelberg’s Medical Microbiology. 23rd Edition, McGraw-Hill/Appleton and Lange, New York.
[3] Todar, K. (2004) Todar’s Online Textbook of Bacteriology: Pseudomonas aeruginosa. Department of Bacteriology, University of Wisconsin, Madison.
[4] Rayner, C.F.J., Cole, P.J. and Wilson, R. (1994) The Management of Chronic Bronchial Sepsis Due to Bronchiectasis. Clinical Pulmonary Medicine, 1, 348-355.
http://dx.doi.org/10.1097/00045413-199411000-00002
[5] Seol, B., Naglic, T., Madic, J. and Bedekovic, M. (2002) In Vitro Antimicrobial Susceptibility of 183 Pseudomonas aeruginosa Strains Isolated from Dogs to Selected Antipseudomonal Agents. Journal of Veterinary Medicine, 49, 188-192.
http://dx.doi.org/10.1046/j.1439-0450.2002.00548.x
[6] Li, X.Z., Livermore, D.M. and Nikaido, H. (1994) Role of Efflux Pump(s) in Intrinsic of Pseudomonas aeruginosa: Resistance to Tetracycline, Chloramphenicol and Norfloxacin. Antimicrob. Antimicrobial Agents and Chemotherapy, 38, 1732-1741.
http://dx.doi.org/10.1128/AAC.38.8.1732
[7] Schaber, J.A., Carty, N.L., MacDonald, N.A., Graham, E.D., Cheluvappa, R., Griswold, J.A. and Hamood, A.N. (2004) Analysis of Quorum Sensing-Dificient Clinical Isolates of Pseudomonas aeruginosa. Journal of Medical Microbiology, 53, 841-853.
http://dx.doi.org/10.1099/jmm.0.45617-0
[8] Wilson, R. and Dowling, R. (1998) Pseudomonas aeruginosa and Other Related Species. Thorax, 53, 231-219.
http://dx.doi.org/10.1136/thx.53.3.213
[9] Benson, H.J. (1998) Antimicrobial Sensitivity Testing: the Kirby-Bauer Method. In: Benson, H.J., Ed., Microbiological Applications: Laboratory Manual in General Microbiology, 7th Edition, McGraw Hill, Boston, 139-141.
[10] (2003) National Committee for Clinical Laboratory Standards, Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard M7-A6, National Committee for Clinical Laboratory Standards, Wayne.
[11] National Committee for Clinical Laboratory Standards, Performance Standards for Antimicrobial Susceptibility Testing (2003) M100-S13. National Committee for Clinical Laboratory Standards, Wayne.
[12] Brodie, S.B., Sands, K.E., Gray, J.E., Parker, R.A., Goldmann, D.A., Davis, R.B. and Richardson, D.K. (2000) Occurrence of Nosocomial Bloodstream Infections in Six Neonatal Intensive Care Units. Pediatric Infectious Disease Journal, 19, 56-65.
http://dx.doi.org/10.1097/00006454-200001000-00012
[13] Abou-dobara, M.I., Deyab, M.A., Elsawy, E.M. and Mohamed, H.H. (2010) Antimicrobial Susceptibility Typing and Genotyping Pattern of Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa Isolated from Urinary Tract Infected Patients. Polish Journal of Microbiology, 59, 207-212.
[14] Kumar, A. and Talwar, A. (2010) Study on the Presence of K. pneumonia and K. oxytocain Raw Milk and Antibiotic Susceptibility Testing. Human Ecology, 32, 207-208.
[15] Visser, M.R., Arska, M.R., Beumer, H., Hoepelman, I.M. and Verhoef, J. (1991) Comparative in Vitro Antibacterial Activity of Sparfloxacin (AT-4140; RP 64206), a New Quinolone. Antimicrobial Agents and Chemotherapy, 35, 858-868.
http://dx.doi.org/10.1128/AAC.35.5.858
[16] Calva, J.J., Sifuentes-Osornio, J. and Ceron, C. (1996) Antimicrobial Resistance in Fecal Flora: Longitudinal Community-Based Surveillance of Children from Urban Mexico. Antimicrobial Agents and Chemotherapy, 40, 1699-1702.
[17] Sack, R.B., Rahman, M., Yunus, M. and Khan, E.H. (1997) Antimicrobial Resistance in Organisms Causing Diarrheal Disease. Clinical Infectious Diseases, 24, S102-S105.
http://dx.doi.org/10.1093/clinids/24.Supplement_1.S102
[18] Hoge, C.W., Gambel, J.M., Srijan, A., Pitarangsi, C. and Echeverria, P. (1998) Trends in Antibiotic Resistance among Diarrheal Pathogens Isolated in Thailand over 15 Years. Clinical Infectious Diseases, 26, 341-345.
http://dx.doi.org/10.1086/516303
[19] Col, N.F. and O’Connor, R.W. (1987) Estimating Worldwide Current Antibiotic Usage: Report of Task Force 1. Clinical Infectious Diseases, 9, S232-S243.
http://dx.doi.org/10.1093/clinids/9.Supplement_3.S232
[20] Kunin, C.M. (1993) Resistance to Antimicrobial Drugs—A Worldwide Calamity. Annals of Internal Medicine, 118, 557-561.
http://dx.doi.org/10.7326/0003-4819-118-7-199304010-00011
[21] Carmeli, Y., Troillet, N., Eliopoulos, G.M. and Samore, M.H. (1999) Emergence of Antibiotic-Resistant Pseudomonas aeruginosa: Comparison of Risks Associated with Different Antipseudomonal Agents. Antimicrobial Agents and Chemotherapy, 43, 1379-1382.
[22] Lee, P.R., Lurie, P., Silverman, M.M. and Lydecker, M. (1991) Drug Promotion and Labeling in Developing Countries: An Update. Journal of Clinical Epidemiology, 45, 49-55.
http://dx.doi.org/10.1016/0895-4356(91)90113-N
[23] Paredes, P., Pena, M., Guerra, E.F., Diaz, J. and Trostle, J. (1996) Factors Influencing Physicians’ Prescribing Behavior Ion the Treatment of Childhood Diarrhea: Knowledge May Not Be the Clue. Social Science & Medicine, 42, 1141-1153.
http://dx.doi.org/10.1016/0277-9536(95)00387-8

Copyright © 2024 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.