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Antioxidant Activity and Lipoxygenase Inhibitory Effect of Caralluma arabica and Related Polyphenolic Constituents

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DOI: 10.4236/ajps.2014.511176    3,878 Downloads   4,939 Views   Citations

ABSTRACT

Phytochemicals have been of great interest as a source of natural antioxidants used for health promotion, food preservation, food flavoring and cosmetics. In this research, alcoholic extract from Caralluma arabica and different solvent fractions were evaluated for their antioxidant, anti-inflammatory and anti-cancer properties as well as their polyphenolic compositions. The total antioxidant property was estimated by the ferric reducing antioxidant power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2, 2-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS+) and β-carotene bleaching tests. Among the ethanol extract and three fractions, the ethyl acetate fraction showed the highest phenolic content (33.1 mg gallic acid/g) and the best antioxidant activity. The ascorbic acid equivalent antioxidant capacities of the ethyl acetate fraction were 143.64, 381.3, 112.6 μmol/g in FRAP, ABTS and DPPH assays, respectively. This study verified that the ethanol extract and ethyl acetate fraction from Caralluma arabica have strong antioxidant activity that was correlated with their high level of phenolic content. Furthermore, the same extract showed appreciable anti-inflammatory via Lipoxygenase inhibitory activity (LOX), the IC50 values ranged from 11.2 - 30.77 μg/mL. Moreover, Ethyl acetate fraction showed the strongest cytotoxic effect (IC50 = 87.55 μg/mL) against MCF-7 breast cancer cell line. These findings suggest that Caralluma arabica may be considered as an interesting source of antioxidants for nutraceutical industries.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Khasawneh, M. , Elwy, H. , Fawzi, N. , Hamza, A. , Chevidenkandy, A. and Hassan, A. (2014) Antioxidant Activity and Lipoxygenase Inhibitory Effect of Caralluma arabica and Related Polyphenolic Constituents. American Journal of Plant Sciences, 5, 1623-1631. doi: 10.4236/ajps.2014.511176.

References

[1] Williams, R.J., Spencer, J.P.E. and Rice-Evans, C. (2004) Flavonoids and Isoflavones (Phytoestrogens): Absorption, Metabolism, and Bioactivity. Flavonoids: Antioxidants or Signalling Molecules? Free Radical Biology and Medicine, 36, 838-849.
http://dx.doi.org/10.1016/j.freeradbiomed.2004.01.001
[2] Soobrattee, M.A., Neergheen, V.S., Luximon-Ramma, A., Aruoma, O.I. and Bahorun, T. (2005) Phenolic as Potential Antioxidant Theraputic Agents: Mechanism and Actions. Mutation Research, 579, 200-213.
[3] Ansari, N.M., Houlihan, L. Hussain, B. and Pieroni, A. (2005) Antioxidant Activity of Five Vegetables Traditionally consumed by South-Asian Migrants in Bradford, Yorkshire, UK. Phytopherapy, 19, 907-911.
[4] Marwah, R.G., Fatope, M.O., Mahrooqi, R., Varma, G.B., Al badi, H. and Al-Burtamani, S.K. (2006) Antioxidant Capacity of Some Edible and Wound Healing Plants in Oman. Food chemistry, 101, 465-470.
http://dx.doi.org/10.1016/j.foodchem.2006.02.001
[5] Zakaria, M.N.M., Islam, M.W., Radhakrishnan, R., Chen, H.B., Kamil, M., Al-Gifri, A.N., Chan, K. and Al-Attas, A. (2001) Anti-Nociceptive and Anti-Inflammatory Properties of Caralluma arabica. Journal of Ethnopharmacology, 76, 155-158.
http://dx.doi.org/10.1016/S0378-8741(01)00208-2
[6] Habibuddin, M., Daghriri, H.A., Humaira, T., Al Qahtani, M.S. and Hefzi, A.A. (2008) Antidiabetic Effect of Alcoholic Extract Caralluma sinaica L. on Streptozotocin-Induced Diabetic Rabbits. Journal of Ethnopharmacology, 117, 215-220.
http://dx.doi.org/10.1016/j.jep.2008.01.021
[7] Tatiya, A.U., Kulkarni, A.S., Surana, S.J. and Bari, N.D. (2010) Antioxidant and Hypolipidemic Effect of Caralluma adscendens Roxb. in Alloxanized Diabetic Rats. International Journal of Pharmacology, 6, 400-406.
http://dx.doi.org/10.3923/ijp.2010.400.406
[8] Al-harbi, M.D., Quershi, S., Raza, M., Ahmed, M.M., Afzal, M. and Shah, A.H. (1994) Evaluation of Caralluma tuberculata Pretreatment for the Protection of Rat Gastric Mucosa against Toxic Damage. Toxicology and Applied Pharmacology, 128, 1-8.
http://dx.doi.org/10.1006/taap.1994.1173
[9] Bader, A., Braca, A., De Tommasi, N. and Morelli, I. (2003) Further Constituents from Caralluma negevensis. Phytopherapy, 62, 1277-1281.
[10] Singleton, V.L., Orthofer, R. and Lamuela-Raventos, R.M. (1999) Analysis of Total Phenols and Other Oxidation Substrates and Antioxidants by Means of Folin-Ciocalteu Reagent. In: Packer, L., Ed., Methods in Enzymology, Academic Press, Harcourt Brace & Company, San Diego, London, New York, Tokyo, 152-178.
[11] Nenadis, N., Lazaridou, O. and Tsimidou, M.Z. (2007) Use of Reference Compounds in Antioxidant Activity Assessment. Journal of Agriculture and Food Chemistry, 55, 5452-5460.
http://dx.doi.org/10.1021/jf070473q
[12] Erel, O. (2004) A Novel Automated Direct Measurement Method for Total Antioxidant Capacity Using a New Generation, More Stable ABTS Radical Cation. Clinic Biochemistry, 37, 277-285.
http://dx.doi.org/10.1016/j.clinbiochem.2003.11.015
[13] Lim, Y.Y. and Quah, E.P.L. (2007) Antioxidant Properties of Different Cultivars of Portulaca oleracea. Food Chemistry, 103, 734-740.
http://dx.doi.org/10.1016/j.foodchem.2006.09.025
[14] Wu, H. (1996) Affecting the Activity of Soybean Lipoxygenase-1. Journal of Molecular Graphics, 14, 331-337.
http://dx.doi.org/10.1016/S0263-7855(97)00006-4
[15] Abad-Garcia, B., Berrueta, L.A., Lopez-Marquez, D.M., Crespo-Ferrer, I., Gallo, B. and Vicente, F. (2007) Optimization and Validation of a Methodology Based on Solvent Extraction and Liquid Chromatography for the Simultaneous Determination of Several Polyphenolic Families in Fruit Juices. Journal of Chromatography, 1154, 87-96.
http://dx.doi.org/10.1016/j.chroma.2007.03.023
[16] Yang, J., Hailiu, R. and Halim, L. (2009) Antioxidant and Antiproliferative Activities of Common Edible Nut Seeds. Food Science and Technology, 42, 1-8.
[17] Conforti, F., Sosa, S., Marrelli, M., Menichini, F., Statti, G.A., Uzunov, D., Tubaro, A., Menichini, F. and Loggia, R.L. (2008) In Vivo Anti-Inflammatory and in Vitro Antioxidant Activities of Mediterranean Dietary Plants. Journal of Ethnopharmacology, 116, 144-151.
http://dx.doi.org/10.1016/j.jep.2007.11.015
[18] Conforti, F., Sosa, S., Marrelli, M., Menichini, F., Statti, G.A., Uzunov, D., Tubaro, A. and Menichini, F. (2009) The Protective Ability of Mediterrannean Dietary Plants against the Oxidative Damage: The Role of Radical Oxygen Species in Inflammation and the Polyphenol, Flavonoid and Sterol Contents. Food Chemistry, 112, 587-594.
http://dx.doi.org/10.1016/j.foodchem.2008.06.013
[19] Wojdylo, A., Oszmianski, J. and Czemerys, R. (2007) Antioxidant Activity and Phenolic Compounds in 32 Selected Herbs. Food Chemistry, 105, 940-949.
http://dx.doi.org/10.1016/j.foodchem.2007.04.038
[20] Rackova, L., Oblozinsky, M., Kastalova, D., Kettmann, V. and Bezakova, L. (2007) Free Radical Scavenging Activity and Lipoxygenase Inhibition of Mahonia aquifolium Extract and Isoquinoline Alkaloids. Journal of Inflammation, 4, 1-7. http://dx.doi.org/10.1186/1476-9255-4-15

  
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