Effect of Feeding Probiotics on Rats' Immunity and Health Conditions during Pregnancy

Abdul-Rahman A. Ali, Ahmed M. M. Metwally, Amal H. Mahmoud, Hadil F. Attia

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DOI: 10.4236/fns.2011.22013                

Abstract

Pregnancy is associated with down regulating cell-mediated immunity (T-Lymphocyte) which would lead for increasing susceptibility to viral and bacterial infection, therefore it is expected that feeding probiotic bacteria would help in strengthening pregnant immunity. To test the effect of probiotics feeding on immunity during pregnancy, rats were fed on four different diets, a basal diet (the control) or basal diet fortified with different probiotics which were yogurt (Streptococcus thermophilus EMCC 1043 and Lactobacillus delbruckii subsp bulgaricus EMCC 1102) (G1), yogurt plus Bifidobacterium breve (G2), and yogurt plus Bifidobacterium breve plus Lactobacillus paracasei (G3). Feeding started before mating, during gestation and after parturition. Probiotics particularly (G2) and (G3) improved signifi-cantly rats' body weight gain over the control during gestation and after parturition and their off-spring. G2 and G3 significantly lowered total serum cholesterol than the control. Low Density Lipoprotein (LDL)-fraction decreased insignificantly during gestation but the difference was significant after parturition. Probiotic feeding enhanced leukocyte production which was differentiated more into lymphocytes than phagocytes particularly on third week of gestation and after parturition. CD4+ counts in the control rats suffered a decrease on pregnancy and the decrease continued during gestation period and after parturition. On the other hand, probiotic feeding helped CD4 to overcome the decrease occurred on pregnancy to give counts on the third week and after parturition higher than before pregnancy. CD8+ counts were also increased with probiotics feeding as compared to the control which suffered continuous decrease during pregnancy. Therefore, probiotics feeding improved pregnant rats' general health, lipid profile and both sides of immunity.

Keywords

Immunity; Lipoproteins; Probiotics; Pregnancy

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	P. Luppi, “How Immune Mechanisms are Affected by Pregnancy,” Vaccine, Vol. 21, No. 24, 2003, pp. 3352- 3357. doi:10.1016/S0264-410X(03)00331-1
[2]	T. E. Ivanova, M. T. S. R. Aivazova, A. Nallansk, T. Chernev and D. S. Kiurkchiev, “Changes of Activation in T-Lymphocyte Subpopulations in Peripheral Blood during Pregnancy,” Akush Ginekol (Sofira), Vol. 46, 2007, pp. 3-8.
[3]	S. J. Orange and Z. K. Ballas, “Natural Killer Cells in Human Health and Disease,” Clinical Immunology, Vol. 118, No. 1, 2006, pp. 1-10. doi:10.1016/j.clim.2005.10.011
[4]	M. M. Faas, A. Bouman, L. Angelique, N. Veenstra, S. Gerda, M. Henk, H. J. Maas and V. Paul, “Species Differences in the Effect of Pregnancy on Lymphocyte Cytokine Production between Human and Rat,” Journal of Leukocyte Biology, Vol. 78, No. 4, 2005, pp. 946-953. doi:10.1189/jlb.0405186
[5]	L. M. Andrew and M. David, “Policing Pregnancy: Tregs Keep the Peace,” Trends in Iimmunology, Vol. 25, 2004, pp. 563-565. doi:10.1016/j.it.2004.09.001
[6]	C. E. Lewis, “Adverse Effect of Pregnancy on High Density Lipoprotein (HDL) Cholesterol in Young Adult Women,” American Journal of Epidemiology, Vol. 144, 1996, pp. 247-254.
[7]	M. V. Tejada-Simon, H. J. Lee, Z. Ustunol and J. J. Pestka, “Ingestion of Yogurt Containing Lactobacillus Acidophilus and Bifidobacterium to Potentiate Immunoglobulin: A Responses to Cholera Toxin in Mice,” Journal of Dairy Science, Vol. 82, 1999, pp. 649-660. doi:10.3168/jds.S0022-0302(99)75281-1
[8]	E. J. Schiffrin, F. Rochat, H. Link-Amster, J. M. Aeschlimann and A. Donnet-Hughes, “Immunomodulation of Human Blood Cells Following the Ingestion of Lactic Acid Bacteria,” Journal of Dairy Science, Vol. 78, No. 3, 1995, pp. 491-497. doi:10.3168/jds.S0022-0302(95)76659-0
[9]	A. S. Akaline, G. Siddik and D. Selmin, “Influence of Yogurt and Acidophilus Yogurt on Serum Cholesterol Levels in Mice,” Journal of Dairy Science, Vol. 80, No. 11, 1996, pp. 2721-2725.
[10]	J. Z. Xiao, S. Kondo, N. Takahashi, K. Miyaji, K. Oshidaf, A. Hiramatsu, K. Iwatsuki, S. Kokubo and A. Hosonof, “Effects of Milk Products Fermented by Bifidobacterium Longum on Blood Lipids in Rats and Healthy Adult Male Volunteers,” Journal of Dairy Science, Vol. 86, No. 7, 2003, pp. 2452-2461. doi:10.3168/jds.S0022-0302(03)73839-9
[11]	E. Laffineu, N. Genetet and J. Leonil, “Immunomodulatory Activity of ?-Casein Permeate Medium Fermented by Lactic Acid Bacteria,” Journal of Dairy Science, Vol. 79, 1996, pp. 2112-2120. doi:10.3168/jds.S0022-0302(96)76585-2
[12]	E. J. Ko, J. S. Goh, B. J. Lee, S. H. Choi and P. H. Kim, “Bifidobacterium Bifidum Exhibits a Lipopolysaccharide-Like Mitogenic Activity for Murine B Lymphocytes,” Journal of Dairy Science, Vol. 82, 1999, pp. 1869 -1876. doi:10.3168/jds.S0022-0302(99)75420-2
[13]	J. Leblank, I. Fliss and C. Matar, “Induction of a Humoral Immune Response Following an Escherichia Coli O157:H7 Infection with an Immunomodulatory Peptidic Fraction Derived from Lactobacillus Helveticus-Fermented Milk,” American Society for Microbiology, Vol. 11, No. 6, 2004, pp. 1171-1181.
[14]	J. G. LeBlanc, C. Matar, J. C. Valdez, J. LeBlanc and G. Perdigon, “Immunomodulating Effects of Peptidic Fractions Issued from Milk Fermented with Lactobacillus Helveticus,” Journal of Dairy Science, Vol. 85, 2001, pp. 2733-2742. doi:10.3168/jds.S0022-0302(02)74360-9
[15]	G. Perdigon, S. Alvarez, M. Rachid, G. Aguero and N. Gobbato, “Immune System Stimulation by Probiotics,” Journal of Dairy Science, Vol. 78, No. 7, 1995, pp. 1597- 1606. doi:10.3168/jds.S0022-0302(95)76784-4
[16]	J. van de Water, C. Keen and M. E. Gershwin, “The Influence of Chronic Yogurt Consumption on Immunity,” Journal of Nutrition, Vol. 129, No. 7, 1999, pp. 1492S- 1495S.
[17]	G. Perdigon, E. Vintini, S. Alvarez, M. Medina and M. Medici, “Study of the Possible Mechanisms Involved in the Mucosal Immune System Activation by Lactic Acid Bacteria,” Journal of Dairy Science, Vol. 82, No. 6, 1999, pp. 1108-1114. doi:10.3168/jds.S0022-0302(99)75333-6
[18]	T. Asahara, K. Shimizu, K. Nomoto, T. Hamabata and Y. Takeda, “Probiotic Bifidobacteria Protect Mice from Lethal Infection with Shiga Toxin-Producing Escherichia Coli O157:H7,” Infection and Immunity, Vol. 72, No. 4, 2004, pp. 2240-2247. doi:10.1128/IAI.72.4.2240-2247.2004
[19]	M. Ogawa, K. Shimizu, K. Nomoto, M. Takahashi, M. Watanuki, T. Tanaka, T. Hamabata, S. Yamasaki and Y. Takeda, “Protective Effect of Lactobacillus Casei Strain Shirota on Shiga Toxin-Producing Escherichia Coli O157: H7 Infection in Infant Rabbits,” Infection and Immunity, Vol. 69, No. 2, 2001, pp. 1101-1108. doi:10.1128/IAI.69.2.1101-1108.2001
[20]	A. Samona and R. K. Robison, “Enumeration of Bifidobacteria in Dairy Products,” Journal of Dairy Technology, Vol. 44, No. 3, 1991, pp. 64-66. doi:10.1111/j.1471-0307.1991.tb00639.x
[21]	S. R. A. Fisher, “Statical method for research worker’s,” Oliver and Void, Vol. 14, 1970, p. 140.
[22]	M. Waller and B. Dunkan, “Role for Symmetric Multiply Composition Problem,” American Science Associates, Vol. 65, 1969, p. 485.
[23]	SPSS for Windows, V. 12.0, SPSS Inc., Copyright?2003, Chicago, IL, USA.
[24]	A. G. Rijhsinghani, S. K. Bhatia, T. Tygrettle and T. Waldschmildt, “Effect of Pregnancy on Thymic T Cells Developed,” American Journal of Reproductive Immunology, Vol. 35, 1996.