Development of New Strategy for Non-Antibiotic Therapy: Dromedary Camel Lactoferrin Has a Potent Antimicrobial and Immunomodulator Effects

Alaa B. Ismael, Salama M. Abd El Hafez, Manal B. Mahmoud, Abdel-Kader A. Elaraby, Hany M. Hassan

Department of Medical Biotechnology, Faculty of Applied Medical Sciences, Taif University, Turaba, KSA;Department of Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.
Department of Medical Microbiology, Faculty of Applied Medical Sciences, Taif University, Turaba, KSA;Immunobiology and Immunopharmacology Unit, Animal Reproduction Research Institute (ARRI), Giza, Egypt.
Holding Company for Biological Products & Vaccines VACSERA, Giza, Egypt.
Immunobiology and Immunopharmacology Unit, Animal Reproduction Research Institute (ARRI), Giza, Egypt.

DOI: 10.4236/aid.2013.34034                

Abstract

The human and bovine lactoferrin have been studied extensively, but very few reports have been published concerning camel lactoferrin (cLf). The present study aimed to isolate cLf and evaluate its efficiency including antimicrobial activity and immunomodulator effects. cLf isolation was attempted from camel milk whey using a cation exchange chromatography by SP-Sepharose. The antimicrobial activity of the isolated cLf was investigated against Staphylococcus aureus (S. aureus), Streptococcus agalactiae (S. agalactiae), Escherichia coli (E. coli) and Pseudomonas aeruginosa (P. aerogenosa) strains. The immune effect of cLf was studied by lymphocyte transformation test. It was found that cLf was separated around molecular weight of 80 kDa and showed significant inhibitory effect against E. coli followed by P. aeruginosa, S. agalactiae and S. aureus. cLf increased lymphocyte transformations mean values in a dose dependant manner. The highest transformations mean value was determined at 50 μg/mL. In conclusion, these results suggest that cLf is a potent natural antimicrobial and novel immunomodulator agent.

 

Keywords

Dromedary Camel Lactoferrin; Isolation; Antimicrobial and Immunomodulator Effects

Conflicts of Interest

The authors declare no conflicts of interest.

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