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Conditional Replication Adenovirus Sensitizes A549 Cancer Cell to Cisplatin

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DOI: 10.4236/eng.2012.410B043    3,233 Downloads   4,094 Views  

ABSTRACT

Objective: The aim of the study was to improve the therapeutic effect for lung cancer using a synergetic strategy of adenovirus-based gene therapy combined with chemotherapy. Methods: A conditional replication adenovirus(CRAd) was employed to treat the A549 lung cancer cells and cisplatin-resisted A549(A549-DDP) cells. In vitro MTS / PMS assay were used to evaluate the cell viability. PCR were used to detect expression of Coxsackie receptor (CAR) and Multidrug resistance(MDR) gene. The in vivo anti-tumor effect of CRAd and cisplatin was evaluated using a subcutaneous mouse model. Results: The CRAd sensitizes A549 cancer cells to cisplatin. The mechanism of enhanced cell growth inhibition is associated with the increased CAR and MDR expression. Conclusion: Our approach is better than the conventional gene therapy and chemotherapy strategy.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Y. Liu and Y. Huang, "Conditional Replication Adenovirus Sensitizes A549 Cancer Cell to Cisplatin," Engineering, Vol. 4 No. 10B, 2012, pp. 165-166. doi: 10.4236/eng.2012.410B043.

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