In Vitro Plant Regeneration of Morus indica L. cv. V1 Using Leaf Explant

Melur Kodandaram Raghunath, Karaba N. Nataraja, Jyothi Sainath Meghana, Radha Sivarajan Sajeevan, Mala V. Rajan, Syed Mashayak Hussaine Qadri

Central Sericultural Research and Training Institute, Srirampura, Mysore, India;.
Department of Crop Physiology, University of Agricultural Sciences, GKVK, Bangalore, India..

DOI: 10.4236/ajps.2013.410249                

Abstract

Adventitious bud induction and plantlet regeneration were studied in a popular mulberry variety, V1 using leaf as an explant. Fully expanded leaf explants were cultured on Murashige and Skoog’s (MS) medium supplemented with thidiazuron (TDZ) (0.5-4.0 mg/l), 6-benzylaminopurine (BAP) (0.5-2.0 mg/l), indole acetic acid (IAA) (2.0 mg/l), gibberlic acid (GA₃) (1.0-2.0 mg/l) silver nitrate (AgNO₃) (2.0 mg/l) and different carbon sources such as sucrose, fructose and glucose (10%-30%) either individually or in combination to induce adventitious buds and regeneration. The highest percentage (63%) of adventitious bud formation and regeneration (68%) was achieved in the medium containing MS with TDZ (1.0 mg/l), IAA (2.0 mg/l) and AgNO₃ (2.0 mg/l). For subsequent regeneration and shoot elongation the MS medium having BAP (1.0 mg/l), GA3 (2.0 mg/l) and AgNO₃ (2.0 mg/l) was found to be suitable. Amongst the carbon sources tested, the most suitable carbon source was found to be sucrose (3%) followed by fructose (2%) for adventitious bud formation. Excised in vitro shoots were rooted (60%-80%) in half strength MS medium supplemented with indole-3-butyric acid (1.0 mg/l). The well rooted plantlets were hardened in soil + sand + farm yard manure (FYM) mixture with a success rate of 70%-90%. Since in vitro regeneration is highly genotype-dependent in mulberry, the standardized protocol can be effectively used for further improvement of this leading genotype using biotechnological approaches.

Keywords

In Vitro Regeneration; Adventitious Bud; Thidiazuron; Mulberry cv. V1; Carbon Source

Conflicts of Interest

The authors declare no conflicts of interest.

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