Supplementation of Vitamin E and C Reduces Oxidative Stress in Broiler Breeder Hens during Summer

B. P. Jena, N. Panda, R. C. Patra, P. K. Mishra, N. C. Behura, B. Panigrahi

De- partment of Livestock Production and Management, College of Veterinary Science and Animal Husbandry, Orissa University of Agriculture and Technology (OUAT), Bhubaneswar, India..
Department of Poultry Science, College of Veterinary Science and Animal Husbandry, Orissa University of Agriculture and Tech- nology (OUAT), Bhubaneswar, India.
Department of Poultry Science, College of Veterinary Science and Animal Husbandry, Orissa University of Agriculture and Tech- nology (OUAT), Bhubaneswar, India;Department of Animal Nutrition, College of Veterinary Science and Animal Husbandry, Orissa University of Agriculture and Technology (OUAT), Bhubaneswar, India.
Department of Veterinary Medicine, College of Veterinary Science and Animal Husbandry, Orissa University of Agriculture and Technology (OUAT), Bhubaneswar, India.

DOI: 10.4236/fns.2013.48A004                

Abstract

The present experiment was carried out to evaluate the effects of vitamin E and C supplementation on the antioxidant status of broiler breeder hens during summer. Two hundred seventy broiler breeder birds (coloured) were divided into nine groups with three replicates having 10 birds in each, and were supplemented two levels of antioxidant vitamins E (250 mg or 500 mg/kg) or C (200 mg or 400 mg/kg) alone or in combinations for a period of 8 weeks. All the vitamin supplemented groups showed significantly lowered malondialdehyde (MDA) level, higher activities of superoxide dismutase (SOD), catalase (CAT) enzymes and higher Ferric Reducing Antioxidant Power (FRAP) activities in erythrocytes than control. However, supplementation with combination of vitamins proved to be better than individual supplementation. It could be concluded that the combination of vitamins C and E at lower level (@ 200 mg/kg and 250 mg/kg, respectively) had higher erythrocytic antioxidant status in coloured broiler breeder hens as compared to individual vitamin supplementation during hot and humid stress.

Keywords

Vitamin E; Vitamin C; Antioxidant Status; Hen; Summer

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	U. Puthpongsiriporn, S. E. Scheideler, J. L. Sell and M. M. Beck, “Effects of Vitamin E and C Supplementation on Performance, in Vitro Lymphocyte Proliferation, and Antioxidant Status of Laying Hens During Heat Stress,” Poultry Science, Vol. 80, No. 8, 2001, pp. 1190-1200.
[2]	A. K. Panda, S. V. Ramarao, M. V. L. N. Raju and R. N. Chatterjee, “Effect of Dietary Supplementation with Vitamins E And C on Production Performance, Immune Responses and Antioxidant Status of White Leghorn Layers under Tropical Summer Conditions,” British Poultry Science, Vol. 49, No. 5, 2008, pp. 592-599. doi:10.1080/00071660802337233
[3]	A. Franchini, M. Canti, G. Manfreda, S. Bertuzzi, G. Asdrubali and C. Franciosi, “Vitamin E as Adjuvant in Emulsified Vaccine for Chicks,” Poultry Science, Vol. 70, No. 8, 1991, pp. 1709-1715. doi:10.3382/ps.0701709
[4]	S. N. Meydani and J. B. Blumberg, “Vitamin E and the Immune Response,” In: S. Cunningham-Rundles, Ed., Nutrient Modulation of the Immune Response, Marcel Dekker, New York, 1993, pp. 223-238.
[5]	K. Sahin, N. Sahin, M. Onderci, S. Yaralioglu and O. Kucuk, “Protective Role of Supplemental Vitamin E on Lipid Peroxidation, Vitamin E, A and Some Mineral Concentrations of Broilers Reared under Heat Stress,” Veterinaria Medicinia—Czech, Vol. 46, No. 5, 2001, pp. 140-144.
[6]	A. K. Panda, S. V. Ramarao and M. V. L. N. Raju, “Effect of Vitamin C Supplementation on Performance, Immune Response and Antioxidant Status of Heat Stressed White Leghron Layers,” Indian Journal of Poultry Science, Vol. 42, No. 2, 2007, pp. 169-173.
[7]	K. Sahin, N. Sahin, M. Onderci, M. F. Gursu and M. Issi, “Vitamin C and E Can Alleviate Negative Effects of Heat Stress in Japanese Quails,” Food, Agriculture and Environment, Vol. 1, No. 2, 2003, pp. 244-249.
[8]	A.O.A.C., “Official Methods of Analysis, Association of Official Analytical Chemists,” 13th Edition, PO Box NO540, Benjamin Franklin Station, Washington DC, 1995.
[9]	Z. A. Placer, L. Cushman and B. Johnson, “Estimation of Products of Lipid Peroxidation (MDA) in Biochemical System,” Analytical Biochemistry, Vol. 16, No. 2, 1966, pp. 359-364. doi:10.1016/0003-2697(66)90167-9
[10]	S. Marklund and G. Marklund, “Involvement of Superoxide Anion Radical in the Autooxidation of Pyrogallol and a Convenient Assay for SOD,” European Journal of Biochemistry, Vol. 47, No. 3, 1974, pp. 464-474. doi:10.1111/j.1432-1033.1974.tb03714.x
[11]	M. Menami and H. Yoshikawa, “Simplified Assay Method of SOD Activity of Clinical Use,” Clinica Chimica Acta, Vol. 92, No. 3, 1979, pp. 337-342. doi:10.1016/0009-8981(79)90211-0
[12]	G. Cohen, D. Dembiec and J. Marens, “Measurement of Catalase Activity in Tissue Extracts,” Analytical Biochemistry, Vol. 34, No. 1, 1970, pp. 30-38. doi:10.1016/0003-2697(70)90083-7
[13]	F. F. Benzie and J. J. Strain, “Ferric Reducing Antioxidant Power Assay: Direct Measure of Total Antioxidant Activity of Biological Fluids and Modified Version of Simultaneous Measurement of Total Antioxidant Power and Ascorbic Acid Concentration,” Methods in Enzymology, Vol. 299, No. 1, 1999, pp. 15-23. doi:10.1016/S0076-6879(99)99005-5
[14]	G. W. Snedecor and W. G. Cochran, “Statistical Methods,” 8th Edition, Lowa State University Press, Ames, lowa, 1994.
[15]	D. B. Duncan, “Multiple Ranges and Multiple F Test,” Biometrics, Vol. 11, No. 1, 1955, pp. 1-42. doi:10.2307/3001478
[16]	K. Sahin, N. Sahin and S. Yaralioglu, “Effects of Vitamin C and Vitamin E on Lipid Peroxidation, Blood Serum Metabolites and Mineral Concentrations of Laying Hens Reared at High Ambient Temperature,” Biological Trace Element Research, Vol. 85, No. 1, 2002, pp. 35-45. doi:10.1385/BTER:85:1:35
[17]	Y. F. Lin, H. L. Tsai, Y. C. Lee and S. J. Chang, “Maternal Vitamin E Supplementation Affects the Antioxidant Capability and Oxidative Status of Hatching Chicks,” Journal of Nutrition, Vol. 135, No. 10, 2005, pp. 24572461.
[18]	N. Sahin, M. Onderci, K. Sahin, M. F. Gursu and M. O. Smith, “Ascorbic Acid and Melatonin Reduce Heat-Induced Performance Inhibition and Oxidative Stress in Japanese Quails,” British Poultry Science, Vol. 45, No. 1, 2004, pp. 116-122. doi:10.1080/00071660410001668941
[19]	K. Z. Mahmoud and A. A. Hijazi, “Effect of Vitamin A and/or E on Plasma Enzymatic Antioxidant Systems and Total Antioxidant Capacity of Broiler Chickens Challenged with Carbon Tetrachloride,” Journal of Animal Physiology and Animal Nutrition, Vol. 91, No. 7-8, 2007, pp. 333-340. doi:10.1111/j.1439-0396.2006.00659.x
[20]	Y. Eid, T. Ebeid, M. Moawad and M. El-Habbak, “Reduction of Dexamethasone-Induced Oxidative Stress and Lipid Peroxidation in Laying Hens by Dietary Vitamin E Supplementation,” Emirates Journal of Food and Agriculture, Vol. 20, No. 2, 2008, pp. 28-40.
[21]	V. Sujatha, J. P. Korde, S. K. Rastogi, S. Maini, K. Ravikanth and D. S. Rekhe, “Amelioration of Heat Stress Induced Disturbances of the Antioxidant Defence System in Broilers,” Journal of Veterinary Medicine and Animal Health, Vol. 2, No. 3, 2010, pp. 18-28.
[22]	R. Ozturk-urek, L. A. Bozkayaa and L. Tarhan, “The Effect of Some Antioxidant Vitamin and Trace ElementSupplemented Diets on Activities of SOD, CAT, GSH-Px and LPO Levels in Chicken Tissues,” Cell Biochemistry Function, Vol. 19, No. 2, 2001, pp. 125-132. doi:10.1002/cbf.905
[23]	S. Yamaguchy, “The Role of SOD an Antioxidant,” Journal of National Cancer Institute, Vol. 28, No. 3, 1991, pp. 221-232.
[24]	H. Yardibi and G. Turkay, “The Effects of Vitamin E on the Antioxidant System, Egg Production, and Egg Quality in Heat Stressed Laying Hens,” Turkish Journal of Veterinary and Animal Science, Vol. 32, No. 5, 2008, pp. 319-325.