Protective Effect of Phenolic-Rich Extracts from Different Parts of Opuntia joconostle Fruit against Carbon Tetrachloride-Induced Oxidative Stress in Mice

Obed Osorio-Esquivel, Alicia Ortiz-Moreno, Julieta Herrera-Martínez, María Dolores Hernández-Navarro

Departamento de Bioingenieria y Biotecnología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México City, México.
Departamento de Farmacia, Facultad de Química, Universidad Autónoma del Estado de México, Toluca, México.
Departamento de Morfología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México City, México.

DOI: 10.4236/jbnb.2013.43A005                

Abstract

Opuntia joconostle fruit is a rich source of biocompounds such as polyphenols including gallic, vanilic, 4-hidroxybenzoic, cafeic, and syringic acids, catechin, epicatechin, rutin, and vanillin, besides betalains. The objective of this research was to evaluate the effect of supplementation polyphenols-rich extracts from different parts of Opuntia joconostle against carbon tetrachloride-induced oxidative stress in a mouse model. The animals were treated orally with polyphenols-rich extracts at 50, 100 and 200 mg/kg BW for 30 consecutive days. On day 30^th the mice received carbon tetrachloride (CCl₄) as hepatoxic agent. Biochemical evaluations were carried out 24 h after induction of the oxidative stress. Data showed that methanolic extracts from different parts of Opuntia joconostle exerting protective effect against the CCl₄-induced oxidative stress in mice. Histology examination revealed that the damage decreased in groups treated with polyphenols-rich extracts compared to the group that did not receive any treatment. Opuntia joconostle fruit contains many phenolic compounds, flavonoids and betalains. The protective effect of extracts may be related to the phenolic composition and also by a counteraction with other compounds, such as betalains and flavonoids that increase their antioxidant effect.

Keywords

Opuntia joconostle; Oxidative Stress; Carbon Tetrachloride; Phenolic Compounds

Conflicts of Interest

The authors declare no conflicts of interest.

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