Improved Micropropagation in Hybanthus enneaspermus L. Muell

Gandhi Premkumar, Neelakantan Arumugam, Sankaralingam Muthuramkumar, Govidaraju Varatharaju, Kaniappan Rajarathinam

Department of Biotechnology, Government College Technology, Coimbatore, India..
Department of Biotechnology, Pondicherry University, Pondicherry, India;.
Department of Botany, Virudhunagar Hindu Nadar Senthikumara Nadar College (Autonomous), Virudhunagar, India.
Department of Botany, Virudhunagar Hindu Nadar Senthikumara Nadar College (Autonomous), Virudhunagar, India;.

DOI: 10.4236/ajps.2013.46144                

Abstract

Multiple shoots were induced on the cut ends of the leaf of Hybanthus enneaspermus on MS medium fortified with different concentration of cytokinins (KI and BAP) and in combination of KI and BAP. To enhance the multiple shoots induction, different types of auxins were used. IAA induced a maximum of 52.3 shoots per explants in combination with KI + BAP. The regenerated shoots show two different morphological leaves. Shoots are 100% rooted on half-strength MS medium supplemented with 2.46 μM·l^-1 IBA. Rooted plantlets were successfully transferred to garden after acclimatization and they appeared morphologically normal.

Keywords

Hybanthus enneaspermus; Multiple Shoots; Regeneration; Morphology

Conflicts of Interest

The authors declare no conflicts of interest.

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