Effects of hyaluronan oligosaccharides on apoptosis ofhuman gingival fibroblasts

Yuki Tanne, Kotaro Tanimoto, Satoru Okuma, Ryo Kunimatsu, Naoto Hirose, Tomomi Mitsuyoshi, Kazuo Tanne

Department of Orthodontics, Applied Life Sciences, Hiroshima University Institute of Biomedical & Health Sciences, Hiroshima, Japan.

DOI: 10.4236/ojst.2013.31004                

Abstract

An increased content of low molecular weight (LMW)- HA was found in human gingiva during the initial phase of periodontitis. A number of functions have been described for LMW-HA in inflammatory processes. It is also demonstrated that LMWHA induces apoptosis in chondrocytes and tumor cells, however, effects of LMWHA on the apoptosis of gingival fibroblasts (GFs) remain unclear. The purpose of this study was to investigate the effects of HA oligosaccharides (HAoligo) on the apoptosis of GFs. GFs were isolated from palatal gingival tissues around the upper first premolars of eight youth (6 -10 years old) and eight adult (26 -35 years old) subjects. Cultured GFs were subjected to the analyses, ELISA for cell proliferation, quantitative real time-PCR for gene expression of Bcl-2, and caspase-3 assay for apoptotic potency. Proliferative activity of cultured GFs was increased significantly

Keywords

Hyaluronan Oligosaccharide; Apoptosis;Caspase-3

Conflicts of Interest

The authors declare no conflicts of interest.

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