Ultradian oscillators of the circadian clock in Saccharomyces cerevisiae

Sara S. Dick, Aya Ryuzoji, Dorothy M. Morré, D. James Morré

Department of Foods and Nutrition, Purdue University, West Lafayette, USA.
Department of Medicinal Chemistry, Purdue University, West Lafayette, USA.

DOI: 10.4236/abc.2013.31008                

Abstract

The yeast, Saccharomyces cerevisiae, has an ENOX1 activity with a period length of 24 min similar to that of other eukaryotes. In contrast to other eukaryotes, however, Saccharomyces cerevisiae has a second ENOX1-like activity with a period length of 25 min. The latter is distinguishable from the traditional ENOX1 on the basis of the longer period length along with resistance to an ENOX1 inhibitor, simalikalactone D, and failure to be phased by melatonin. In addition, two periods are apparent in measurements of oxygen consumption indicating that the consumption of oxygen to water occurs independently by homodimers of both of the two forms of ENOX. Based on the measurements of glyceraldehyde-3- phosphate dehydrogenase, S. cerevisiae exhibits circadian activity maxima at 24 and 25 h together with a 40 h period possibly representing the 40 min metabolic rhythm of yeast not observed in our measurement of oxygen consumption and normally observed only with continuous cultures. The findings are indicative of at least three independent time-keeping systems being operative in a single cell.

Keywords

Biological Clock; ECTO-NOX (ENOX) Proteins; Ultradian Oscillator; Yeast (Saccharomyces cerevisiae)

Conflicts of Interest

The authors declare no conflicts of interest.

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