Green Tea Is a Poor Contributor to Tissue Folate in a Folate Depletion-Repletion Rat Model

Kaori Endoh, Michael Fenech, Keizo Umegaki

CSIRO Food and Nutritional Sciences, Adelaide, Australia.
Division of Nutritional Sciences, School of Food and Nutritional Sciences, University of Shizuoka, Shizuoka, Japan.
Information Center, National Institute of Health and Nutrition, Tokyo, Japan..

DOI: 10.4236/fns.2013.42019                

Abstract

Green tea contains folate and epigallocatechin gallate (EGCg), which is suggested to be an antifolate. In this study, we examined whether green tea was a good source of folate in a folate depletion/repletion rat model. Rats fed a folate deficient diet for 4 weeks were supplied with folate for 7 days, and then folate repletion effects were evaluated in terms of increase in total folate level in plasma, liver, and bone marrow and decrease in plasma homocysteine level. In this model, the folic acid treatment effect was observed to be dose-dependent and an appropriate dose of folic acid was 40 μg/kg/day or greater. Based on this finding, green tea as well as spinach, chicken liver, and folic acid as a reference were applied to this model. Increase in tissue folate level in response to the food samples varied among tissues, with bone marrow showing the smallest response. Increase in tissue folate level was in the order of spinach > chicken liver > green tea, which produced only a slight increase in tissue folate level and further diminished bone marrow folate level. EGCg administered by intragastric gavage at an approximate dose of 8 mg/kg did not attenuate the increase in tissue folate level when repletion was performed with folic acid. These results suggested that green tea is a poor source of food folate, but EGCg in green tea at a low dose has little effect on folic acid absorption.

Keywords

Green Tea; Food Folate; Folic Acid; Epigallocatechin Gallate; Rat Model

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Food and Nutrition Board, “Dietary Reference Intakes for Thiamin, Riboflavin, Niacin, Vitamin B6, Folate, Vitamin B12, Pantothenic Acid, Biotion, and Choline,” National Academic Press, Washington DC, 1998.
[2]	L. D. Botto, C. A. Moore, M. J. Khoury and J. D. Erickson, “Neural-Tube Defects,” The New England Journal of Medicine, Vol. 341, No. 20, 1999, pp. 1509-1519. doi:10.1056/NEJM199911113412006
[3]	M. Fenech, “Biomarkers of Genetic Damage for Cancer Epidemiology,” Toxicology, Vol. 181-182, 2002, pp. 411-416. doi:10.1016/S0300-483X(02)00480-8
[4]	L. B. Bailey, G. C. Rampersaud and G. P. Kauwell, “Folic Acid Supplements and Fortification Affect the Risk for Neural Tube Defects, Vascular Disease and Cancer: Evolving Science,” Journal of Nutrition, Vol. 133, No. 6, 2003, pp. 1961S-1968S.
[5]	J. F. Gregory, “The Bioavailability of Folate,” In: L. B. Bailey, Ed., Folate in Health and Disease, M. Dekker, New York, 1995, pp. 195-235.
[6]	M. J. Whitrow, V. M. Moore, A. R. Rumbold and M. J. Davies, “Effect of Supplemental Folic Acid in Pregnancy on Childhood Asthma: A Prospective Birth Cohort Study,” American Journal of Epidemiology, Vol. 170, No. 12, 2009, pp. 1486-1493. doi:10.1093/aje/kwp315
[7]	Y. Clement, “Can Green Tea Do That? A Literature Review of the Clinical Evidence,” Preventive Medicine, Vol. 49, No. 2-3, 2009, pp. 83-87. doi:10.1016/j.ypmed.2009.05.005
[8]	N. Imaeda, C. Goto, Y. Tokudome, M. Ikeda, S. Maki and S. Tokudome, “Folate Intake and Food Sources in Japanese Female Dietitians,” Environmental Health and Preventive Medicine, Vol. 7, No. 4, 2002, pp. 156-161. doi:10.1007/BF02897944
[9]	N. C. Alemdaroglu, S. Wolffram, J. P. Boissel, E. Closs, H. Spahn-Langguth and P. Langguth, “Inhibition of Folic Acid Uptake by Catechins and Tea Extracts in Caco-2 Cells,” Planta Medica, Vol. 73, No. 1, 2007, pp. 27-32. doi:10.1055/s-2006-951745
[10]	E. Navarro-Peran, J. Cabezas-Herrera, F. Garcia-Canovas, M. C. Durrant, R. N. Thorneley and J. N. Rodriguez-Lopez, “The Antifolate Activity of Tea Catechins,” Cancer Research, Vol. 65, No. 6, 2005, pp. 2059-2064. doi:10.1158/0008-5472.CAN-04-3469
[11]	M. Shiraishi, M. Haruna, M. Matsuzaki, E. Ota, R. Murayama and S. Murashima, “Association between the Serum Folate Levels and Tea Consumption during Pregnancy,” BioScience Trends, Vol. 4, No. 5, 2010, pp. 225-230.
[12]	N. C. Alemdaroglu, U. Dietz, S. Wolffram, H. Spahn-Langguth and P. Langguth, “Influence of Green and Black Tea on Folic Acid Pharmacokinetics in Healthy Volunteers: Potential Risk of Diminished Folic Acid Bioavailability,” Biopharmaceutics & Drug Disposition, Vol. 29, No. 6, 2008, pp. 335-348. doi:10.1002/bdd.617
[13]	K. Augustin, J. Frank, S. Augustin, P. Langguth, V. Ohrvik, C. M. Witthoft, G. Rimbach and S. Wolffram, “Green Tea Extracts Lower Serum Folates in Rats at Very High Dietary Concentrations Only and Do Not Affect Plasma Folates in a Human Pilot Study,” Journal of Physiology and Pharmacology, Vol. 60, No. 3, 2009, pp. 103-108.
[14]	A. J. Clifford, N. D. Bills, J. M. Peerson, H. G. Muller, G. E. Burk and K. D. Rich, “A Depletion-Repletion Folate Bioassay Based on Growth and Tissue Folate Concentrations of Rats,” Journal of Nutrition, Vol. 123, No. 5, 1993, pp. 926-932.
[15]	K. Umegaki and T. Ichikawa, “Decrease in Vitamin E Levels in the Bone Marrow of Mice Receiving Whole-Body X-Ray Irradiation,” Free Radical Biology & Medicine, Vol. 17, No. 5, 1994, pp. 439-444. doi:10.1016/0891-5849(94)90170-8
[16]	K. Endoh, M. Murakami, R. Araki, C. Maruyama and K. Umegaki, “Low Folate Status Increases Chromosomal Damage by X-Ray Irradiation,” International Journal of Radiation Biology, Vol. 82, No. 4, 2006, pp. 223-230. doi:10.1080/09553000600721817
[17]	B. Frick, K. Schrocksnadel, G. Neurauter, B. Wirleitner, E. Artner-Dworzak and D. Fuchs, “Rapid Measurement of Total Plasma Homocysteine by HPLC,” Clinica Chimica Acta, Vol. 331, No. 1-2, 2003, pp. 19-23. doi:10.1016/S0009-8981(03)00076-7
[18]	K. Aiso and T. Tamura, “Trienzyme Treatment for Food Folate Analysis: Optimal pH and Incubation Time for Alpha-Amylase and Protease Treatment,” Journal of Nutritional Science and Vitaminology (Tokyo), Vol. 44, No. 3, 1998, pp. 361-370. doi:10.3177/jnsv.44.361
[19]	K. Umegaki, A. Sugisawa, K. Yamada and M. Higuchi, “Analytical Method of Measuring Tea Catechins in Human Plasma by Solid-Phase Extraction and HPLC with Electrochemical Detection,” Journal of Nutritional Science and Vitaminology (Tokyo), Vol. 47, No. 6, 2001, pp. 402-408. doi:10.3177/jnsv.47.402
[20]	A. J. Clifford, M. K. Heid, J. M. Peerson and N. D. Bills, “Bioavailability of Food Folates and Evaluation of Food Matrix Effects with a Rat Bioassay,” Journal of Nutrition, Vol. 121, No. 4, 1991, pp. 445-453.
[21]	M. Matsuzaki, M. Haruna, E. Ota, S. Sasaki, Y. Nagai and S. Murashima, “Dietary Folate Intake, Use of Folate Supplements, Lifestyle Factors, and Serum Folate Levels among Pregnant Women in Tokyo, Japan,” Journal of Obstetrics and Gynaecology Research, Vol. 34, No. 6, 2008, pp. 971-979. doi:10.1111/j.1447-0756.2008.00821.x
[22]	M. J. Derelanko, “Determination of Erythrocyte Life Span in F-344, Wistar, and Sprague-Dawley Rats Using a Modification of the [3H]Diisopropyl-Fluorophosphate ([3H]DFP) Method,” Fundamental and Applied Toxicology, Vol. 9, No. 2, 1987, pp. 271-276. doi:10.1093/toxsci/9.2.271
[23]	G. J. Ward and P. F. Nixon, “Modulation of Pteroylpolyglutamate Concentration and Length in Response to Altered Folate Nutrition in a Comprehensive Range of Rat Tissues,” Journal of Nutrition, Vol. 120, No. 5, 1990, pp. 476-484.
[24]	J. Hamelet, K. Demuth, J. Dairou, A. Ledru, J. L. Paul, J. M. Dupret, J. M. Delabar, F. Rodrigues-Lima and N. Janel, “Effects of Catechin on Homocysteine Metabolism in Hyperhomocysteinemic Mice,” Biochemical and Biophysical Research Communications, Vol. 355, No. 1, 2007, pp. 221-227. doi:10.1016/j.bbrc.2007.01.142
[25]	S. Wang, S. K. Noh and S. I. Koo, “Green Tea Catechins Inhibit Pancreatic Phospholipase A(2) and Intestinal Absorption of Lipids in Ovariectomized Rats,” The Journal of Nutritional Biochemistry, Vol. 17, No. 7, 2006, pp. 492-498. doi:10.1016/j.jnutbio.2006.03.004
[26]	T. Yoshikawa, H. Yamada, K. Matsuda, H. Nhno, Y. M. Sagesaka, T. Kakuda, K. Toyoizumi, K. Matsumoto, K. Kosuge, S. Uchida, S. Onoue, S. Yamada and K. Umegaki, “Effects of Short-Term Consumption of a Large Amount of Tea Catechins on Chromosomal Damage, Oxidative Stress Markers, Serum Lipid, Folic Acid, and Total Homocysteine Levels: A Randomized, Double-Blind, Controlled Study,” Japanese Journal of Clinical Pharmacology and Therapeutics, Vol. 43, No. l, 2012, pp. 9-16. doi:10.3999/jscpt.43.9
[27]	K. Umegaki, T. Esashi, M. Tezuka, A. Ono, A. Sano and I. Toimita, “Determination of Tea Catechins in Food by HPLC with an Electrochemical Detector,” Food Hygiene and Safety Science, Vol. 37, No. 2, 1996, pp. 77-82. doi:10.3358/shokueishi.37.77
[28]	M. Eichholzer, O. Tonz and R. Zimmermann, “Folic Acid: A Public-Health Challenge,” Lancet, Vol. 367, No. 9519, 2006, pp. 1352-1361. doi:10.1016/S0140-6736(06)68582-6
[29]	R. Ye, A. Ren, L. Zhang, Z. Li, J. Liu, L. Pei and X. Zheng, “Tea Drinking as a Risk Factor for Neural Tube Defects in Northern China,” Epidemiology, Vol. 22, No. 4, 2011, pp. 491-496. doi:10.1097/EDE.0b013e31821b4526