Development of Different Strategies for the Clean-Up of Polychlorinated Biphenyls (PCBs) Congeners Using Pressurized Liquid Extraction

Amal Al-Rashdan, Murad I. H. Helaleh

Kuwait Institute for Scientific Research, Deputy Director General Office for Research, Safat, Kuwait.

DOI: 10.4236/jep.2013.41011                

Abstract

The extracted fish samples (w/w) using pressurized liquid extraction (PLE) were cleaned up by applying different adsorbent materials such as: combination of acidic/ basic silica and alumina, florisil, and silica gel either alone or in combination with another sort of sorbent. Different solvents such as hexane (H), dichloromethane (DCM), with different compositions were used to elute the PCBs target compounds from the spiked fish samples. Overall the mean percentage recoveries for all PCBs congeners using PLE were ranging from 78.6% to 98.7%; from 79.4% to 91.8%; from 65.8% to 104.5% and from 82.4% to 100.9% for cases A, B, C, D and E, respectively. However, the overall mean percentage recoveries for the 13C-PCBs surrogate including: 101, 138, 153, 180 and 209 were ranging from 88.2% to 97.6%; from 79.4% to 90.2%, from 88.3% to 96.4%, from 79.8% to 90.4% and from 83.9% to 95.9%, for cases A, B, C, D and E, respectively. The limits of detection of the proposed method were varied from 0.01 to 0.28 ng/g (w/w) for different PCBs congeners. The proposed method has linear dynamic range from 21.5 ng/g to 109 ng/g. This method was applied to fish samples for the determination of the target PCBs components.

Keywords

Polychlorinated Biphenyls (PCBs); Pressurized Liquid Extraction (PLE); Adsorbent Materials; Gel Permeation Chromatography (GPC); Negative Chemical Ionization (NCI); Marine Sample

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	F. Wania and D. Mackay, “Tracking the Distribution of Persistent Organic Pollutant,” Environmental Science and Technology, Vol. 30, No. 9, 1996, pp. 390A-396A. doi:10.1021/es962399q
[2]	E. Bjorklund, T. Nilson and S. Bowadt, “Pressurized Li quid Extraction of Persistent Organic Pollutants in Environmental Analysis,” Trends in Analytical Chemistry, Vol. 19, No. 7, 2000, pp. 434-445. doi:10.1016/S0165-9936(00)00002-9
[3]	H. Giergielewicz-Mozajska, L. Dabrowski and J. Namiesnik, “Accelerated Solvent Extraction (ASE) in the Analysis of Environmental Solid Samples-Some Aspects of Theory and Practice,” Critical Review in Analytical Chemistry, Vol. 31, No. 3, 2001, pp. 149-165. doi:10.1080/20014091076712
[4]	A. Muller, E. Bjorklund and C. Von Holst, “On-Line Clean-Up of Pressurized Liquid Extracts for the Determination of Polychlorinated Biphenyls in Feedingstuffs and Food Matrices Using Gas Chromatography-Mass Spectrometry,” Journal of Chromatography A, Vol. 925, No. 1-2, 2001, pp. 197-205. doi:10.1016/S0021-9673(01)01028-7
[5]	E. Bjorklund, A. Muller and C. Von Holst, “Comparison of Fat Retainers in Accelerated Solvent Extraction for the Selective Extraction of PCBs from Fat-Containing Samples,” Analytical Chemistry, Vol. 73, No. 16, 2001, pp. 4050-4053. doi:10.1021/ac010178j
[6]	S. Sporring and E. Bjorklund, “Selective Pressurized Li quid Extraction of Polychlorinated Biphenyls from Fat Containing Food and Feed Samples: Influence of Cell Dimensions, Solvent Type, Temperature and Flush Volume,” Journal of Chromatography A, Vol. 1040, No. 2, 2004, pp. 155-161. doi:10.1016/j.chroma.2004.04.022
[7]	B. E. Richter, “Current Trends and Developments in Sam ple Preparation,” LCGC, Vol. 17, No. 6S, 1999, pp. S22 S28.
[8]	USEPA Method, “Pressurized Liquid Extraction Test Methods for Evaluating Solid Waste,” US Environmental Protection Agency US Environmental Protection Agency, Vol. 3545, Washington DC, 1995.
[9]	M. M. Schantz, J. J. Nichols and S. A. Wise, “Evaluation of Pressurized Fluid Extraction for the Extraction of Environmental Matrix Reference Materials,” Analytical Chemistry, Vol. 69, No. 20, 1997, pp. 4210-4219. doi:10.1021/ac970299c
[10]	B. E. Richter, B. A. Jones, J. L. Ezzel, N. L. Porter, N. Avdalovic and C. Pohl, “Accelerated Solvent Extraction: A Technique for Sample Preparation,” Analytical Chemistry, Vol. 68, No. 6, 1996, pp. 1033-1039. doi:10.1021/ac9508199
[11]	J. J .L. F. Moreno, F. J. A. Liebanas, A. G. Frenich and J. L. M. Vidal, “Evaluation of Different Sample Treatments for Determining Pesticide Residues in Fat Vegetable Matrices like Avocado by Low-Pressure Gas Chromatography-Tandem Mass Spectrometry,” Journal of Chromatography A, Vol. 1111, No. 1, 2006, pp. 97-105. doi:10.1016/j.chroma.2006.01.108
[12]	P. Suchan, J. Pulkrabova, J. Hajslova and V. Kocourek, “Pressurized Liquid Extraction in Determination of Po lychlorinated Biphenyls and Organochlorinated Pesticides in Fish Samples,” Analytica Chimica Acta, Vol. 520, No. 1-2, 2004, pp. 193-200. doi:10.1016/j.aca.2004.02.061
[13]	K. Kitamura, Y. Takazawa, S. Hashimoto. J.-W. Choi, H. Ito and M. Morita, “Effective Extraction Method for Dioxin Analysis from Lipid-Rich Biological Matrices Using a Combination of Pressurized Liquid Extraction and Dimethyl Sulfoxide/Acetonitrile/Hexane Partitioning,” Analytica Chimica Acta, Vol. 512, No. 1, 2004, pp. 27-37. doi:10.1016/j.aca.2004.02.040
[14]	E. Martinez, M. Gros, S. Lacorte and D. Barcelo, “Simplified Procedures for the Analysis of Polycyclic Aroma tic Hydrocarbons in Water, Sediments and Mussels,” Journal of Chromatography A, Vol. 1047, No. 2, 2004, pp. 181 188.
[15]	K. Saito, A. Sjodin, C. D. Sandau, M. D. Davis, H. Nakazawa, Y. Matsuki and D. G. Patterson Jr., “Development of a Accelerated Solvent Extraction and Gel Permeation Chromatography Analytical Method for Measuring Persistent Organohalogen Compounds in Adipose and Organ Tissue Analysis,” Chemosphere, Vol. 57, No. 5, 2004, pp. 373-381. doi:10.1016/j.chemosphere.2004.04.050
[16]	Q. Jiang, N. Hanari, Y. Miyake, T. Okazawa, R. K. F. Lau, K. Chen, B. Wyrzykowska, M. K. So, N. Yamashitab and P. K. S. Lam, “Health Risk Assessment for Polychlorinated Biphenyl, Polychlorinated Dibenzo-p-Dioxins and Dibenzofurans, and Polychlorinated Naphthalenes in Seafood from Guangzhou and Zhoushan, China,” Environmental Pollutant, Vol. 148, No. 1, 2007, pp. 31-39. doi:10.1016/j.envpol.2006.11.002
[17]	K. S. Kumar, K. Kannan, J. P. Giesy and S. Masunaga, “Distribution and Elimination of Polychlorinated Diben zo-p-Dioxins, Dibenzofurans, Biphenyls, and p,p’-DDE in Tissues of Bald Eagles from the Upper Peninsula of Michigan,” Environmental Science and Technology, Vol. 36, No. 13, 2002, pp. 2789-2796. doi:10.1021/es0114660
[18]	M. K. So, X. Zhaug, J. P. Giesy, C. N. Fung, H. W. Fong, J. Zheng, M. J. Kramer, H. Yoo and P. K. S. Lam, “Organochlorines and Dioxin-Like Compounds in Green Lipped Mussels Perna viridis from Hong Kong Mariculture Zones,” Marine Pollutant Bulletin, Vol. 51, No. 8-12, 2005, pp. 677-687. doi:10.1016/j.marpolbul.2005.06.004
[19]	S. Jayaraman, R. J. Pruell and R. Mc Kinney, “Extraction of Organic Contaminants from Marine Sediments and Tissues Using Microwave Energy,” Chemosphere, Vol. 44, No. 2, 2001, pp. 181-191. doi:10.1016/S0045-6535(00)00201-0
[20]	A. Hubrt, K. D. Wenzel, W. Engelworld and G. Shuurmann, “Accelerated Solvent Extraction—More Efficient Extraction of POPs and PAHs from Real Contaminated Plant and Soil Samples,” Reviewers in Analytical Chemistry, Vol. 20, No. 2, 2001, pp. 101-144.
[21]	V. R. Meyer, “Practical High-performance liquid chromatography”, 2nd Edition, Frankfurt, 1996.
[22]	M. Numata, T. Yarita, Y. Aoyagi, Y. Tsuda, M. Yamazaki, A. Takatsu, K. Ishikawa, K. Chiba and K. Okamaoto, “Sediment Certified Reference Materials for the Determination of Polychlorinated Biphenyls and Organochlorine Pesticides from the National Metrology Institute of Japan (NMIJ),” Analytical Bioanalytical Chemistry, Vol. 387, No. 7, 2007, pp. 2313-2323. doi:10.1007/s00216-006-0691-z
[23]	J. J. Ramos, C. Dietz, M. J. Gonzalez and L. Ramos, “Miniaturized Selective Pressurized Liquid Extraction of Po lychlorinated Biphenyls from Foodstuffs,” Journal of Chromatography A, Vol. 1152, No. 1-2, 2007, pp. 254-261. doi:10.1016/j.chroma.2006.11.097
[24]	U. N. Fidalgo, G. E. Blanco and M. A. Sanz, “Sample Handling Strategies for the Determination of Persistent Organic Contaminants from Biota Samples,” Analytica Chimica Acta, Vol. 590, No. 1, 2007, pp. 1-16. doi:10.1016/j.aca.2007.03.004
[25]	M. I. H. Helaleh and A. Al-Rashdan, “Application of Automated Pressurized Liquid Extraction (PLE) and Powerprep Clean-Up System for the Analysis of Polyhydrocarbons, Organochlorinated Pesticides and Polychlo rinated Biphenyls in Marine Samples,” Analytical Methods, 2012, In Press.
[26]	Murad I. H. Helaleh, Amal Al-Rashdan and A. Ibtisam, “Simultaneous Analysis of Organochlorinated Pesticides (OCPs) and Polychlorinated Biphenyls (PCBs) from Marine Samples Using Automated Pressurized Liquid Extraction (PLE) and Power PrepTM Clean-Up,” Talanta, Vol. 94, 2012, pp. 44-49. doi:10.1016/j.talanta.2012.02.031
[27]	N. Yang, M. Matsuda, M. Kawano and T. Wakimoto, “PCBs and Organochlorine Pesticides (OCPs) in Edible Fish and Shellfish from China,” Chemosphere, Vol. 63, No. 8, 2006, pp. 1342-1352. doi:10.1016/j.chemosphere.2005.09.029