In vitro antagonistic potential of Streptomyces sp. AM-S1 against plant and human pathogens

Kandhasamy Sowndhararajan, Sun Chul Kang

Department of Biotechnology, College of Engineering, Daegu University, Gyeongsan, South Korea.

DOI: 10.4236/jacen.2012.11007                

Abstract

In the present investigation, a total number of 132 different actinomycetes strains were isolated from the humus soil samples. Out of 132 isolates, 52 showed inhibitory activity against the fungal pathogen Rhizoctonia solani. Among the antagonists, the isolate designated as AM-S1 exhibited maximum inhibitory activity against the test pathogen R. solani (41 mm). Further, the light microscopic observations of the co-cultures showed severe structural alterations in the mycelia of R. solani near the zone of inhibition. The isolate AM-S1 was identified as Streptomyces sp. by morphological and 16S rDNA sequence analysis. The color of the aerial and substrate mycelia produced by the Streptomyces sp. AM-S1 varied with different media. The isolate Streptomyces sp. AM-S1 also effectively inhibited the growth of various plant and human pathogens. Further works are needed on optimization of this strain’s antagonistic activity, isolation and characterization of the antimicrobial metabolite.

Keywords

Actinomycetes; Streptomyces sp. AM-S1; Antagonistic Activity; Rhizoctonia solani

Conflicts of Interest

The authors declare no conflicts of interest.

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