Experimental Results of the Fibrin Clot Use to Accelerate the Regeneration of Damaged Bone in the Rat Lower Jaw

Igor Maiborodin, Andrey Shevela, Tatyana Perrin, Ivan Kolesnikov, Vera Matveeva, Alexander Shevela, Boris Sheplev, Irina Kolmakova

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DOI: 10.4236/ss.2010.11001                

Abstract

Morphological and radiological methods were used to study regeneration of the damaged bone of rat mandibles after application of plateletenriched fibrin clot. A bone hole was artificially created, and in the natural course of regeneration, the hole was immediately filled with blood and there a blood clot formed. After one week of healing, separate islands of young bone tissue appeared. After two to three weeks, the opening in the mandible was completely replaced by the young bone tissue. When a similar bone hole was filled with autological fibrin clot, the blood clot did not form. But after one week the entire hole was filed with newlyformed fused bone tissue. By the second week after the use of fibrin clot, the bone hole had further healed and bone callus was formed.

Keywords

Fibrin Clot, Regeneration of Bone, Density of Bone Tissue

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	M. E. Laidmae, J. L. McCormick, J. J. Herod, T. Pastore, E. S. Salum, P. A. Sawyer, Janmey and R. Uibo, “Stability, Sterility, Coagulation, and Immunologic Studies of Salmon Coagulation Proteins with Potential Use for Mammalian Wound Healing and Cell Engineering,” Biomaterials, Vol. 27, No. 34, 2006, pp. 5771-5779.
[2]	M. Valbonesi, “Fibrin Glues of Human Origin,” Best Practice & Research Clinical Haematology, Vol. 19, No. 1, 2006, pp. 191-203.
[3]	G. Carter, A. Goss, J. Lloyd and R. Tocchetti, “Tranexamic Acid Mouthwash Versus Autologous Fibrin Glue in Patients Taking Warfarin Undergoing Dental Extractions: A Randomized Prospective Clinical Study,” Journal of Oral and Maxillofacial Surgery, Vol. 61, No. 12, 2003, pp. 1432-1435.
[4]	W. D. Spotnitz and R. Prabhu, “Fibrin Sealant Tissue Adhe-sive—Review and Update,” Journal of Long-Term Effects of Medical Implants, Vol. 15, No. 3, 2005, pp. 245-270.
[5]	W. Becker, “Fibrin Sealants in Implant and Periodontal Treat-ment: Case Presentations,” Compend Contin Educ Dent, Vol. 26, No. 8, 2005, pp. 539-545.
[6]	J. Choukroun, A. Diss, A. Simonpieri, M. O. Girard, C. Schoeffler, S. L. Dohan, A. J. Dohan, J. Mouhyi and D. M. Dohan, “Platelet-Rich Fibrin (PRF): A Second-Ge- neration Platelet Concentrate. Part IV: Clinical Effects on Tissue Heal-ing,” Oral Surgery, Oral Medicine, Oral Pathology, Oral Ra-diology, Vol. 101, No. 3, 2006, pp. e56- e60.
[7]	M. B. Schmidt, E. H. Chen and S. E. Lynch, “A Review of the Effects of Insulin-Like Growth Factor and Platelet Derived Growth Factor on in Vivo Cartilage Healing and Repair,” Os-teoarthritis Cartilage, Vol. 14, No. 5, 2006, pp. 403-412.
[8]	D. Schwartz-Arad, L. Levin and M. Aba, “The Use of Platelet Rich Plasma (PRP) and Platelet Rich Fibrin (PRF) Extracts in Dental Implantology and Oral Surgery,” Refuat Hapeh Veha-shinayim, Vol. 24, No. 1, 2007, pp. 51-55, 84.
[9]	E. L. Kaijzel, P. Koolwijk, M. G. vanErck, V. W. vanHinsbergh and M. P. deMaat, “Molecular Weight Fibrinogen Variants Determine Angiogenesis Rate in a Fibrin Matrix in Vitro And in Vivo,” Journal of Thrombosis and Haemostasis, Vol. 4, No. 9, 2006, pp. 1975-1981.
[10]	S. McDougall, J. Dallon, J. Sherratt and P. Maini, “Fibroblast Migration and Collagen Deposition During Dermal Wound Healing: Mathematical Modelling and Clinical Implications,” Philosophical Transactions Mathe- matical Physical and Engi-neering Sciences, Vol. 364, No. 1843, 2006, pp. 1385-1405.
[11]	I. V. Maiborodin, I. S. Kolesnikov, B. V. Sheplev, T. M. Ra-gimova, A. I. Shevela, A. N. Kovyntsev, I. A. Komakova, I. A. Pritchina, E. V. Kozlova, A. B. Voitovich and D. N. Kovyntsev, “Granulomatous Inflammation after Use Fibrin Preparation,” Morphological Letters, No. 3-4, 2007, pp. 116-118.
[12]	I. V. Maiborodin, I. S. Kolesnikov, B. V. Sheplev and T. M. Ragimova, “Application of Fibrin and its Preparations in Sto-matology,” Stomatologiia (Mosk), Vol. 87, No. 6, 2008, pp. 75-77. I. V. Maiborodin, I. S. Kolesnikov, B. V. Sheplev, T. M. Ra-gimova, A. N. Kovyntsev, D. N. Kovyntsev and A. I. Shevela, “Adjusting Gingival Tissues Morphology After Dental Implan-tation with Fibrin Use,” Stomatologiia (Mosk), Vol. 88, No. 1, 2009, pp. 9-13.
[13]	S. J. Froum, S. S. Wallace, D. P. Tarnow and S. C. Cho, “Effect of Platelet-Rich Plasma on Bone Growth and Osseointegration in Human Maxillary Sinus Grafts: Three Bilateral Case Reports,” The International Journal of Periodontics & Restor-ative Dentistry, Vol. 22, No. 1, 2002, pp. 45-53.
[14]	G. Fuerst, R. Gruber, S. Tangl, F. Sanroman and G. Watzek, “Effects of Fibrin Sealant Protein Concentrate with and Without Platelet-Released Growth Factors on Bony Healing of Cortical Mandibular Defects, an Experimental Study in Minipigs,” Clinical Oral Implants Research, Vol. 15, No. 3, 2004, pp. 301-307.
[15]	R. M. London, F. A. Roberts, D. A. Baker, M. D. Rohrer and R. B. O’Neal, “Histologic Comparison of a Thermal Dual-Etched Implant Surface to Machined, TPS, and HA Surfaces: Bone Contact in Vivo in Rabbits,” The International Journal of Oral & Maxillofacial Implants, Vol. 17, No. 3, 2002, pp. 369-376.
[16]	J. Choukroun, A. Diss, A. Simonpieri, M. O. Girard, C. Schoeffler, S. L. Dohan, A. J. Dohan, J. Mouhyi and D. M. Dohan, “Platelet-Rich Fibrin (PRF): A Second-Gen- eration Platelet Concentrate. Part V: Histologic Evaluations of PRF Effects on Bone Allograft Maturation in Sinus Lift,” Oral Sur-gery, Oral Medicine, Oral Pathology, Oral Radiology & En-dodontics, Vol. 101, No. 3, 2006, pp. 299-303.
[17]	D. M. Dohan, J. Choukroun, A. Diss, S. L. Dohan, A. J. Dohan, J. Mouhyi and B. Gogly, “Platelet-Rich Fibrin (PRF): A Second-Generation Platelet Concentrate. Part III: Leucocyte Activation: A New Feature for Platelet Concentrates?” Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology & Endodontics, Vol. 101, No. 3, 2006, pp. e51-e55.
[18]	S. Kellouche, S. Mourah, A. Bonnefoy, D. Schoevaert, M. P. Podgorniak, F. Calvo, M. F. Hoylaerts, C. Legrand and C. Dosquet, “Platelets, Thrombospondin-1 and Human Dermal Fibroblasts Cooperate for Stimulation of Endothelial Cell Tu-bulogenesis through VEGF and PAI-1 Regulation,” Experi-mental Cell Research, Vol. 313, No. 3, 2007, pp. 486-499.
[19]	K. Ito, Y. Yamada, T. Naiki and M. Ueda, “Simultaneous Im-plant Placement and Bone Regeneration Around Dental Implants Using Tissue-Engineered Bone with Fibrin Glue, Mesenchymal Stem Cells and Platelet-Rich Plasma,” Clinical Oral Implants Research, Vol. 17, No. 5, 2006, pp. 579-586.