Studies on Processing and Characterization of Hydroxyapatite Biomaterials from Different Bio Wastes

Sudip Mondal, Biswanath Mondal, Apurba Dey, Sudit S. Mukhopadhyay

Centre for Advanced Material Processing, Central Mechanical Engineering Research Institute,Mahatma Gandhi Avenue, Durgapur-713209, India.
Department of Biotechnology, National Institute of Technology, Mahatma Gandhi Avenue Durgapur-713209, India.

DOI: 10.4236/jmmce.2012.111005                

Abstract

Development of suitable materials that acts as an interface between the implant and tissues in body system structurally, mechanically and bio functionally is important for the success of tissue engineering. This motivated materials scientists and biologists to find out suitable bioactive materials for the aforementioned purpose. There has been growing interest in developing bioactive synthetic ceramics that could closely mimic natural apatite characteristics. Hydroxyapatite (HAp) has been widely used as a biocompatible ceramic but mainly for contact with bone tissue, due to its resemblance to mineral bone. This study presents the synthesis and characterization of HAp materials from different sources like bovine bone and fish scales and their application in tissue engineering. The phase purity and crystallinity of different calcined HAp powder was determined by XRD and FTIR analysis. The Thermo Gravimetric and Differential Thermal Analysis were carried out to show the thermal stability of the HAp powder. The morphology of the powder was observed under Scanning Electron Microscopy (SEM). Cytotoxicity evaluation of the developed powder was carried out in RAW macrophage like cell line media for an incubation period of 72 hours. These results proved the biocompatibility of HAp powders obtained from different biosources for tissue engineering applications.

Keywords

Hydroxyapatite; Biomaterials; Tissue Engineering; Cytotoxicity;

Conflicts of Interest

The authors declare no conflicts of interest.

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