The CD133/1⁺ cell subset from human subcutaneous adult fat retains hemogenic potential

Camila Santos de Moraes, Paulo Roberto Albuquerque Leal, Daniel Fabiano Ferreira, Fernando Serra, Eliana Abdelhay, Claudia Sondermann Freitas

Bone Marrow Transplantation Unit, National Cancer Institute of Rio de Janeiro, Rio de Janeiro, Brazil.
Experimental Medicine Program, Research Coordination, National Cancer Institute of Rio de Janeiro, Rio de Janeiro, Brazil.
Pedro Ernesto Hospital, Rio de Janeiro State University, Rio de Janeiro, Brazil.
Plastic Surgery, Cancer Hospital I, National Cancer Institute of Rio de Janeiro, Rio de Janeiro, Brazil.

DOI: 10.4236/scd.2012.22006                

Abstract

Research has shown that cells from adult fat tissue can effect long-term blood reconstitution. Fat-derived multipotentiality was ascribed to CD34⁺ perivascular populations from its prominent microvasculature, that represent mostly non-hemogenic, mesenchymal cells, although this tissue contains a CD34⁺45⁺ subset committed to a hemogenic fate. Here, in order to analyze cell subsets presenting hemogenic capabilities within fat, CD133/1⁺ and pericytes, the latter defined by CD140b (PDGFRb, Platelet-Derived Growth Factor Receptor Beta) expression, were immunomagnetically selected from stromal-vascular fractions (SVF). In Vitro Colony Forming Unit (CFU) assays were negative for CD140b⁺ pericytes and positive for CD133/1⁺ cells when a prolonged CFU assay was performed, revealing fat as another store of primitive progenitors that retain hemogenic potential.

Keywords

Adipose Tissue-Derived Stem Cells; CD133; Hematogenesis; Hemangioblast; Pericyte

Conflicts of Interest

The authors declare no conflicts of interest.

References

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