Background: Lesbian, Gay and Bisexual (LGB) are individuals who often experienced stigma and discrimination which has detrimental effects on psychological well-being. Objectives: This study aimed to identify the major stressors and psychological well-being of LGB among Chinese adults in Hong Kong. Methods: A cross-sectional study was employed using self-administered questionnaires. Convenience sampling was used to recruit subjects. Demographic information, Measure of Gay-related stress (MOGS) and The Center for Epidemiologic Studies Depression Scale (CES-D Scale) were used to collect data. Results: The major stressor among LGB individuals in Hong Kong was family reaction. Majority of them had a high level of depression. Conclusion: LGB individuals in Hong Kong experienced high level of depression.