Author(s)

Talal Aburjai, Muhammed Alzweiri, Yusuf M. Al-Hiari

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Temperature and pressure were shown to vary significantly with solvent mixing, showing maxima at differ-ent solvent ratios. Acetonitrile/water mixing resulted in temperature reduction of solutions whereas metha-nol/water mixing caused temperature increases. On the other hand, maximum recorded chromatographic pressure of acetonitrile:water mixtures occurred at a solvent ratio of 1:6 compared with methanol:water, which showed a maximum pressure at a solvent ratio of 1:1. These findings can be of use in stabilizing re-tention time shifts during HPLC-based studies associated with compound identification based on retention time such as analysis of complex mixtures.

Retention Time, Pressure, Temperature, Methanol and Acetonitrile

T. Aburjai, M. Alzweiri and Y. Al-Hiari, "Temperature and Pressure Behaviours of Methanol, Acetonitrile/Water Mixtures on Chromatographic Systems," American Journal of Analytical Chemistry, Vol. 2 No. 8, 2011, pp. 934-937. doi: 10.4236/ajac.2011.28108.