Author(s)

Robert E. Hoagland^1*, C. Douglas Boyette², Robin H. Jordan¹, Kenneth C. Stetina²

¹Crop Production Systems Research Unit, USDA-ARS, Stoneville, MS, USA.
²Biological Control of Pests Research Unit, USDA-ARS, Stoneville, MS, USA.

Previously we found that a strain of Myrothecium verrucaria (MV) exhibited bioherbicidal activity against several important weeds, and that some commercial formulations of glyphosate applied with MV resulted in synergistic interactions that improved weed control efficacy. We also found that MV had bioherbicidal activity against glyphosate-resistant Palmer amaranth. We have also reported that some commercial formulations are inhibitory to MV. Our objectives were to test the effect of unformulated glyphosate (high purity, technical-grade glyphosate) alone and in combination with MV for bioherbicidal activity on glyphosate-susceptible and -resistant Palmer amaranth biotypes under greenhouse conditions and to examine technical-grade glyphosate on the growth of this bioherbicide. High purity glyphosate (without adjuvants/surfactants) was not toxic to MV growth and sporulation at concentrations up to 2.0 mM when grown on agar supplemented with the herbicide. Both biotypes were injured by MV and MV plus glyphosate treatments as early as 19 h after application (3 h after a dew period of 16 h). These injury effects increased and were more evident through the 6-day time course, when after 120 h the MV plus glyphosate treatment had killed all glyphosate-susceptible and -resistant plants. The interaction of glyphosate plus MV was synergistic toward the control of Palmer amaranth. Data strongly suggest that the active ingredient is responsible for the synergy previously found when this bioherbicide was combined with some commercial formulations of glyphosate. Results demonstrated that MV can control both glyphosate-resistant and -susceptible Palmer amaranth seedlings and act synergistically with high-purity glyphosate to provide improved weed control.

Amaranthus palmeri, Biocontrol Agent, Bioherbicide, Biological Weed Control, Glyphosate-Resistance, Myrothecium verrucaria, Palmer Amaranth, Pigweed

Hoagland, R. , Boyette, C. , Jordan, R. and Stetina, K. (2018) Interaction of the Bioherbicide Myrothecium verrucaria with Technical-Grade Glyphosate on Glyphosate-Susceptible and -Resistant Palmer Amaranth. American Journal of Plant Sciences, 9, 2306-2319. doi: 10.4236/ajps.2018.911167.