Author(s)

Hernández-Marín David Alejandro^1,2,3, Muñoz-Ortega Martín Humberto², Guevara-Lara Fidel³, Gúzman-Lucio Marco Antonio⁴, Sánchez-García Eduardo^1*

¹Laboratorio de Química Analítica, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, Nuevo León, México.
²Laboratorio de Patología Molecular Experimental, Departamento de Química, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Aguascalientes, México.
³Laboratorio de Biotecnología y Funcionalidad de Alimentos, Departamento de Química, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Aguascalientes, México.
⁴Departamento de Botánica, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, México.

Acinetobacter baumannii is one of the most prevalent pathogens in nosocomial infections and has been cause of concern in recent years because, it has presented multiresistance to antibiotics and besides can form biofilms on biotic and abiotic surfaces like tissues and medical devices. Therefore, the search for new alternatives of natural origin to inhibit biofilm formation is being conducted. In the present investigation, sub-lethal concentrations (5.61 mg/mL, 3.74 mg/mL and 1.87 mg/mL) of methanolic extract of Nothoscordum bivalve were evaluated, showing biofilm formation inhibition up to 40.8%, in one nosocomial isolated of A. baumannii by the microtiter biofilm formation assay using crystal violet. On the other hand, the concentrations of 5.61 mg/mL and 3.74 mg/mL, caused an overexpression (up to 15.4 times) in the genes involved in the formation of biofilm (abaI, bap and csuE); due to this, the interaction of the extract with the bacteria was analyzed by scanning electron microscopy (SEM) and cellular damage was observed in the structure and stability of biofilm.

Biofilm, Sub-Lethal, Nothoscordum bivalve, Acinetobacter baumannii

Alejandro, H. , Humberto, M. , Fidel, G. , Antonio, G. and Eduardo, S. (2018) Inhibition of Acinetobacter baumannii Biofilm Formation by Methanolic Extract of Nothoscordum bivalve. Advances in Microbiology, 8, 422-438. doi: 10.4236/aim.2018.85028.