In Vitro Culture of Shell Gland Epithelial Cells in Japanese Quail (Coturnix coturnix japonica) - Open Journal of Animal Sciences

OJAS > Vol.8 No.2, April 2018

Open Journal of Animal Sciences

Volume 8, Issue 2 (April 2018)

ISSN Print: 2161-7597 ISSN Online: 2161-7627

Google-based Impact Factor: 1.39 Citations h5-index & Ranking

In Vitro Culture of Shell Gland Epithelial Cells in Japanese Quail (Coturnix coturnix japonica) ()

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Download as PDF (Size: 610KB) PP. 184-190

DOI: 10.4236/ojas.2018.82013 744 Downloads 1,448 Views Citations

Author(s)

Osamu Kai, Kaoru Mineo

Affiliation(s)

Department of Animal Science and Resources, College of Bioresource Sciences, Nihon University, Fujisawa, Japan.

ABSTRACT

To investigate the secretion of protoporphyrin IX (PpIX), superficial eggshell pigment, from shell gland cells of Japanese quail, the epithelial cells of the gland were collected and isolated for cultivation in vitro. An analysis of a peak for PpIX in the cells was performed using a fluorescence microplate reader. The measurement showed that PpIX has a peak of excitation wavelength at 410 nm and emission wavelength at 606 nm in the culture medium (HamF12 + 4% HCl). Volumes of PpIX in the medium after 4 hour culture of the cells were measured with a microplate reader using filter set of excitation wavelength 400/30nm and emission wavelength 620/40nm. However the cells did not secrete significantly PpIX during 4 hour incubation in this culture system, addition of quail plasma to the medium resulted in significantly higher secretion. A cultivation system in this study is able to use for the study on the mechanism of the secretion of eggshell pigment, PpIX from Japanese quail shell gland epithelial cells.

KEYWORDS

Japanese Quail, Shell Gland Epithelial Cells, In Vitro Culture, Protoporphyrin, Fluorescence Microplate Reader

Share and Cite:

Kai, O. and Mineo, K. (2018) In Vitro Culture of Shell Gland Epithelial Cells in Japanese Quail (Coturnix coturnix japonica). Open Journal of Animal Sciences, 8, 184-190. doi: 10.4236/ojas.2018.82013.

Cited by

[1]	Effects of Zinc on Cell Proliferation, Zinc Transport, and Calcium Deposition in Primary Endometrial Epithelial Cells of Laying Hens In Vitro
	2021

[2]	Calcium Participates in Secretion of Porphyrin from Shell Gland Epithelial Cells of Japanese Quail (Coturnix coturnix japonica)
	2019

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