Metabolomics Profile of Potato Tubers after Phosphite Treatment - American Journal of Plant Sciences

AJPS > Vol.9 No.4, March 2018

American Journal of Plant Sciences

Volume 9, Issue 4 (March 2018)

ISSN Print: 2158-2742 ISSN Online: 2158-2750

Google-based Impact Factor: 1.20 Citations h5-index & Ranking

Metabolomics Profile of Potato Tubers after Phosphite Treatment ()

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DOI: 10.4236/ajps.2018.94065 1,027 Downloads 2,390 Views Citations

Author(s)

Xingxi Gao^1,2, Steven Locke³, Junzeng Zhang⁴, Jyoti Joshi¹, Gefu Wang-Pruski^1*

Affiliation(s)

¹Department of Plant, Food, and Environmental Sciences, Faculty of Agriculture, Dalhousie University, Truro, Canada.
²Department of Edible Fungi Science, Faculty of Agriculture, Ludong University, Yantai, China.
³Aquatic and Crop Resource Development Research Centre, National Research Council of Canada, Charlottetown, Canada.
⁴Aquatic and Crop Resource Development Research Centre, National Research Council of Canada, Halifax, Canada.

ABSTRACT

Phosphite (Phi)-based fungicides are used to control the oomycete Phytophthora infestans which causes late blight disease, the most devastating disease in potatoes. In order to examine the effects of Phi-based fungicides on potato tubers through foliar or post-harvest application, a metabolite profiling approach based on gas chromatography coupled to mass spectrometry (GC-MS) has been established. A total of 132 metabolites were detected using the GC-MS approach. Among these, 34 metabolites were identified after normalization and annotated with a compound name with standard mass spectral library. Metabolomic analysis of Phi-treated plants showed significant differences in the levels of many metabolites especially amino acids. Multivariate statistical approaches, such as principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA), were employed to explore the relationships between metabolites to detect group differences. A good discrimination between the control and the Phi-treated plants was observed, which demonstrated that significant changes in the metabolite profile have been caused by the two different Phi applications (foliar or post-harvest). This finding suggests that the alteration of specific metabolite levels by accumulation of Phi can lead to resistance against the pathogen.

KEYWORDS

Potato, Metabolomics, GC-MS, Phosphite, Phytophthora infestans

Share and Cite:

Gao, X. , Locke, S. , Zhang, J. , Joshi, J. and Wang-Pruski, G. (2018) Metabolomics Profile of Potato Tubers after Phosphite Treatment. American Journal of Plant Sciences, 9, 845-864. doi: 10.4236/ajps.2018.94065.

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[3]	aDepartment of Horticulture and Landscaping, Faculty of Agronomy, Vietnam National University of Agriculture, Hanoi, Vietnam, bGraduate School of Medical …
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